| pubmed-article:12092834 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12092834 | lifeskim:mentions | umls-concept:C0019236 | lld:lifeskim |
| pubmed-article:12092834 | lifeskim:mentions | umls-concept:C0031881 | lld:lifeskim |
| pubmed-article:12092834 | lifeskim:mentions | umls-concept:C0332583 | lld:lifeskim |
| pubmed-article:12092834 | lifeskim:mentions | umls-concept:C0303920 | lld:lifeskim |
| pubmed-article:12092834 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:12092834 | pubmed:dateCreated | 2002-7-2 | lld:pubmed |
| pubmed-article:12092834 | pubmed:abstractText | A green fluorescent protein-labeled fluobody was designed to develop a simple immunoassay method for detecting picloram herbicide in an environmental sample. The gfp gene was successfully inserted into the pSJF2 vector harboring the picloram-specific antibody fragment to yield pSJF2GFP. Picloram spiking in an environmental river sample could be indirectly detected by observing the fluorescence intensity value of the gfp-fluobody, exhibiting specific sensitivity to free picloram with an IC50 value of 50 ppb. Using the gfp-fluobody immunoassay avoids the enzyme-substrate reaction for calorimetric detection that is required in an enzyme-linked immunosorbent assay (ELISA). | lld:pubmed |
| pubmed-article:12092834 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12092834 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12092834 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12092834 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12092834 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12092834 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12092834 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12092834 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12092834 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12092834 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12092834 | pubmed:month | May | lld:pubmed |
| pubmed-article:12092834 | pubmed:issn | 0916-8451 | lld:pubmed |
| pubmed-article:12092834 | pubmed:author | pubmed-author:KiddA SAS | lld:pubmed |
| pubmed-article:12092834 | pubmed:author | pubmed-author:TrevorsJack... | lld:pubmed |
| pubmed-article:12092834 | pubmed:author | pubmed-author:KimIn SeonIS | lld:pubmed |
| pubmed-article:12092834 | pubmed:author | pubmed-author:ShimJae HanJH | lld:pubmed |
| pubmed-article:12092834 | pubmed:author | pubmed-author:SuhYong... | lld:pubmed |
| pubmed-article:12092834 | pubmed:author | pubmed-author:YauKerrm Y... | lld:pubmed |
| pubmed-article:12092834 | pubmed:author | pubmed-author:HallJ... | lld:pubmed |
| pubmed-article:12092834 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12092834 | pubmed:volume | 66 | lld:pubmed |
| pubmed-article:12092834 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12092834 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12092834 | pubmed:pagination | 1148-51 | lld:pubmed |
| pubmed-article:12092834 | pubmed:dateRevised | 2004-12-15 | lld:pubmed |
| pubmed-article:12092834 | pubmed:meshHeading | pubmed-meshheading:12092834... | lld:pubmed |
| pubmed-article:12092834 | pubmed:meshHeading | pubmed-meshheading:12092834... | lld:pubmed |
| pubmed-article:12092834 | pubmed:meshHeading | pubmed-meshheading:12092834... | lld:pubmed |
| pubmed-article:12092834 | pubmed:meshHeading | pubmed-meshheading:12092834... | lld:pubmed |
| pubmed-article:12092834 | pubmed:meshHeading | pubmed-meshheading:12092834... | lld:pubmed |
| pubmed-article:12092834 | pubmed:meshHeading | pubmed-meshheading:12092834... | lld:pubmed |
| pubmed-article:12092834 | pubmed:meshHeading | pubmed-meshheading:12092834... | lld:pubmed |
| pubmed-article:12092834 | pubmed:meshHeading | pubmed-meshheading:12092834... | lld:pubmed |
| pubmed-article:12092834 | pubmed:meshHeading | pubmed-meshheading:12092834... | lld:pubmed |
| pubmed-article:12092834 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12092834 | pubmed:articleTitle | Green fluorescent protein-labeled recombinant fluobody for detecting the picloram herbicide. | lld:pubmed |
| pubmed-article:12092834 | pubmed:affiliation | Department of Chemical and Materials Engineering, University of Alberta, Edmonton, Canada. ikim@ualberta.ca | lld:pubmed |
| pubmed-article:12092834 | pubmed:publicationType | Journal Article | lld:pubmed |
