| pubmed-article:12084772 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12084772 | lifeskim:mentions | umls-concept:C0086045 | lld:lifeskim |
| pubmed-article:12084772 | lifeskim:mentions | umls-concept:C0288250 | lld:lifeskim |
| pubmed-article:12084772 | lifeskim:mentions | umls-concept:C0332157 | lld:lifeskim |
| pubmed-article:12084772 | lifeskim:mentions | umls-concept:C0444504 | lld:lifeskim |
| pubmed-article:12084772 | lifeskim:mentions | umls-concept:C0205359 | lld:lifeskim |
| pubmed-article:12084772 | lifeskim:mentions | umls-concept:C1373200 | lld:lifeskim |
| pubmed-article:12084772 | lifeskim:mentions | umls-concept:C1705175 | lld:lifeskim |
| pubmed-article:12084772 | lifeskim:mentions | umls-concept:C1882348 | lld:lifeskim |
| pubmed-article:12084772 | lifeskim:mentions | umls-concept:C1705178 | lld:lifeskim |
| pubmed-article:12084772 | lifeskim:mentions | umls-concept:C0521116 | lld:lifeskim |
| pubmed-article:12084772 | lifeskim:mentions | umls-concept:C1705176 | lld:lifeskim |
| pubmed-article:12084772 | lifeskim:mentions | umls-concept:C0205374 | lld:lifeskim |
| pubmed-article:12084772 | lifeskim:mentions | umls-concept:C1546465 | lld:lifeskim |
| pubmed-article:12084772 | lifeskim:mentions | umls-concept:C1705177 | lld:lifeskim |
| pubmed-article:12084772 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:12084772 | pubmed:dateCreated | 2002-6-26 | lld:pubmed |
| pubmed-article:12084772 | pubmed:abstractText | Ca(2+) sparks are small, localized cytosolic Ca(2+) transients due to Ca(2+) release from sarcoplasmic reticulum through ryanodine receptors. In smooth muscle, Ca(2+) sparks activate large conductance Ca(2+)-activated K(+) channels (BK channels) in the spark microdomain, thus generating spontaneous transient outward currents (STOCs). The purpose of the present study is to determine experimentally the level of Ca(2+) to which the BK channels are exposed during a spark. Using tight seal, whole-cell recording, we have analyzed the voltage-dependence of the STOC conductance (g((STOC))), and compared it to the voltage-dependence of BK channel activation in excised patches in the presence of different [Ca(2+)]s. The Ca(2+) sparks did not change in amplitude over the range of potentials of interest. In contrast, the magnitude of g((STOC)) remained roughly constant from 20 to -40 mV and then declined steeply at more negative potentials. From this and the voltage dependence of BK channel activation, we conclude that the BK channels underlying STOCs are exposed to a mean [Ca(2+)] on the order of 10 microM during a Ca(2+) spark. The membrane area over which a concentration > or =10 microM is reached has an estimated radius of 150-300 nm, corresponding to an area which is a fraction of one square micron. Moreover, given the constraints imposed by the estimated channel density and the Ca(2+) current during a spark, the BK channels do not appear to be uniformly distributed over the membrane but instead are found at higher density at the spark site. | lld:pubmed |
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| pubmed-article:12084772 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12084772 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12084772 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:12084772 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12084772 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:12084772 | pubmed:issn | 0022-1295 | lld:pubmed |
| pubmed-article:12084772 | pubmed:author | pubmed-author:TuftRichard... | lld:pubmed |
| pubmed-article:12084772 | pubmed:author | pubmed-author:FogartyKevin... | lld:pubmed |
| pubmed-article:12084772 | pubmed:author | pubmed-author:WalshJohn... | lld:pubmed |
| pubmed-article:12084772 | pubmed:author | pubmed-author:ZhugeRonghuaR | lld:pubmed |
| pubmed-article:12084772 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12084772 | pubmed:volume | 120 | lld:pubmed |
| pubmed-article:12084772 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12084772 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12084772 | pubmed:pagination | 15-27 | lld:pubmed |
| pubmed-article:12084772 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:12084772 | pubmed:meshHeading | pubmed-meshheading:12084772... | lld:pubmed |
| pubmed-article:12084772 | pubmed:meshHeading | pubmed-meshheading:12084772... | lld:pubmed |
| pubmed-article:12084772 | pubmed:meshHeading | pubmed-meshheading:12084772... | lld:pubmed |
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| pubmed-article:12084772 | pubmed:meshHeading | pubmed-meshheading:12084772... | lld:pubmed |
| pubmed-article:12084772 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12084772 | pubmed:articleTitle | Spontaneous transient outward currents arise from microdomains where BK channels are exposed to a mean Ca(2+) concentration on the order of 10 microM during a Ca(2+) spark. | lld:pubmed |
| pubmed-article:12084772 | pubmed:affiliation | Biomedical Imaging Group, Department of Physiology, University of Massachusetts Medical School, Worcester, MA 01655, USA. | lld:pubmed |
| pubmed-article:12084772 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12084772 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:12084772 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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