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pubmed-article:12055832pubmed:dateCreated2002-6-11lld:pubmed
pubmed-article:12055832pubmed:abstractTextLeishmania species of the subgenus Viannia account for 88% of all cases of leishmaniasis recorded in Colombia. Correct diagnosis is essential as infection with members of this subgenus can produce disfiguring destruction of the mucosa. Several methods are available to diagnose leishmaniasis in clinical samples. More recently, the polymerase chain reaction (PCR) has been used, with varying sensitivities and specificities depending on the primers used. In this paper we report on the sensitivity and specificity of PCR primers B1/B2 used on clinical samples and compare their use to the conventional parasitological methods. PCR alone is more sensitive than any single conventional method used, but a combination of conventional methods produced comparable sensitivity. PCR is well suited for use in selected cases and as a test for mucosal leishmaniasis.lld:pubmed
pubmed-article:12055832pubmed:languageenglld:pubmed
pubmed-article:12055832pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
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pubmed-article:12055832pubmed:statusMEDLINElld:pubmed
pubmed-article:12055832pubmed:monthAprlld:pubmed
pubmed-article:12055832pubmed:issn0035-9203lld:pubmed
pubmed-article:12055832pubmed:authorpubmed-author:BarkerDouglas...lld:pubmed
pubmed-article:12055832pubmed:authorpubmed-author:RestrepoMarco...lld:pubmed
pubmed-article:12055832pubmed:authorpubmed-author:IsazaDiana...lld:pubmed
pubmed-article:12055832pubmed:authorpubmed-author:ArboledaMarga...lld:pubmed
pubmed-article:12055832pubmed:authorpubmed-author:McCannSharon...lld:pubmed
pubmed-article:12055832pubmed:issnTypePrintlld:pubmed
pubmed-article:12055832pubmed:volume96 Suppl 1lld:pubmed
pubmed-article:12055832pubmed:ownerNLMlld:pubmed
pubmed-article:12055832pubmed:authorsCompleteYlld:pubmed
pubmed-article:12055832pubmed:paginationS165-8lld:pubmed
pubmed-article:12055832pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:12055832pubmed:year2002lld:pubmed
pubmed-article:12055832pubmed:articleTitleValidation of the polymerase chain reaction for the diagnosis of human cutaneous leishmaniasis in north-west Colombia.lld:pubmed
pubmed-article:12055832pubmed:affiliationInstituto Colombiano de Medicina Tropical Antonio Roldan Betancur, Medellin, Colombia.lld:pubmed
pubmed-article:12055832pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:12055832pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
pubmed-article:12055832pubmed:publicationTypeValidation Studieslld:pubmed