| pubmed-article:12054812 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12054812 | lifeskim:mentions | umls-concept:C0035668 | lld:lifeskim |
| pubmed-article:12054812 | lifeskim:mentions | umls-concept:C0680730 | lld:lifeskim |
| pubmed-article:12054812 | lifeskim:mentions | umls-concept:C1609982 | lld:lifeskim |
| pubmed-article:12054812 | lifeskim:mentions | umls-concept:C0024485 | lld:lifeskim |
| pubmed-article:12054812 | lifeskim:mentions | umls-concept:C0231881 | lld:lifeskim |
| pubmed-article:12054812 | lifeskim:mentions | umls-concept:C0678594 | lld:lifeskim |
| pubmed-article:12054812 | lifeskim:mentions | umls-concept:C1948027 | lld:lifeskim |
| pubmed-article:12054812 | lifeskim:mentions | umls-concept:C1148554 | lld:lifeskim |
| pubmed-article:12054812 | lifeskim:mentions | umls-concept:C1516050 | lld:lifeskim |
| pubmed-article:12054812 | lifeskim:mentions | umls-concept:C0439831 | lld:lifeskim |
| pubmed-article:12054812 | lifeskim:mentions | umls-concept:C0205372 | lld:lifeskim |
| pubmed-article:12054812 | lifeskim:mentions | umls-concept:C0521115 | lld:lifeskim |
| pubmed-article:12054812 | lifeskim:mentions | umls-concept:C1171366 | lld:lifeskim |
| pubmed-article:12054812 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:12054812 | pubmed:dateCreated | 2002-6-10 | lld:pubmed |
| pubmed-article:12054812 | pubmed:abstractText | We report a new residual dipolar couplings (RDCs) based NMR procedure for rapidly determining RNA tertiary structure demonstrated on a uniformly (15)N/(13)C-labeled 27 nt variant of the trans-activation response element (TAR) RNA from HIV-I. In this procedure, the time-consuming nuclear Overhauser enhancement (NOE)-based sequential assignment step is replaced by a fully automated RDC-based assignment strategy. This approach involves examination of all allowed sequence-specific resonance assignment permutations for best-fit agreement between measured RDCs and coordinates for sub-structures in a target RNA. Using idealized A-form geometries to model Watson-Crick helices and coordinates from a previous X-ray structure to model a hairpin loop in TAR, the best-fit RDC assignment solutions are determined very rapidly (<five minutes of computational time) and are in complete agreement with corresponding NOE-based assignments. Orientational constraints derived from RDCs are used simultaneously to assemble sub-structures into an RNA tertiary conformation. Through enhanced speeds of application and reduced reliance on chemical shift dispersion, this RDC-based approach lays the foundation for rapidly determining RNA conformations in a structural genomics context, and may increase the size limit of RNAs that can be examined by NMR. | lld:pubmed |
| pubmed-article:12054812 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12054812 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12054812 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12054812 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12054812 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12054812 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12054812 | pubmed:month | May | lld:pubmed |
| pubmed-article:12054812 | pubmed:issn | 0022-2836 | lld:pubmed |
| pubmed-article:12054812 | pubmed:author | pubmed-author:Al-HashimiHas... | lld:pubmed |
| pubmed-article:12054812 | pubmed:author | pubmed-author:GosserYuyingY | lld:pubmed |
| pubmed-article:12054812 | pubmed:author | pubmed-author:GorinAndreyA | lld:pubmed |
| pubmed-article:12054812 | pubmed:author | pubmed-author:MajumdarAnany... | lld:pubmed |
| pubmed-article:12054812 | pubmed:author | pubmed-author:PatelDinshaw... | lld:pubmed |
| pubmed-article:12054812 | pubmed:copyrightInfo | c) 2002 Elsevier Science Ltd. | lld:pubmed |
| pubmed-article:12054812 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12054812 | pubmed:day | 3 | lld:pubmed |
| pubmed-article:12054812 | pubmed:volume | 318 | lld:pubmed |
| pubmed-article:12054812 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12054812 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12054812 | pubmed:pagination | 637-49 | lld:pubmed |
| pubmed-article:12054812 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:12054812 | pubmed:meshHeading | pubmed-meshheading:12054812... | lld:pubmed |
| pubmed-article:12054812 | pubmed:meshHeading | pubmed-meshheading:12054812... | lld:pubmed |
| pubmed-article:12054812 | pubmed:meshHeading | pubmed-meshheading:12054812... | lld:pubmed |
| pubmed-article:12054812 | pubmed:meshHeading | pubmed-meshheading:12054812... | lld:pubmed |
| pubmed-article:12054812 | pubmed:meshHeading | pubmed-meshheading:12054812... | lld:pubmed |
| pubmed-article:12054812 | pubmed:meshHeading | pubmed-meshheading:12054812... | lld:pubmed |
| pubmed-article:12054812 | pubmed:meshHeading | pubmed-meshheading:12054812... | lld:pubmed |
| pubmed-article:12054812 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12054812 | pubmed:articleTitle | Towards structural genomics of RNA: rapid NMR resonance assignment and simultaneous RNA tertiary structure determination using residual dipolar couplings. | lld:pubmed |
| pubmed-article:12054812 | pubmed:affiliation | Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA. hashimi@sbnmr1.mskcc.org | lld:pubmed |
| pubmed-article:12054812 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12054812 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:12054812 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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