| pubmed-article:12046690 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12046690 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:12046690 | lifeskim:mentions | umls-concept:C0044602 | lld:lifeskim |
| pubmed-article:12046690 | lifeskim:mentions | umls-concept:C0126732 | lld:lifeskim |
| pubmed-article:12046690 | lifeskim:mentions | umls-concept:C0028778 | lld:lifeskim |
| pubmed-article:12046690 | lifeskim:mentions | umls-concept:C1334877 | lld:lifeskim |
| pubmed-article:12046690 | lifeskim:mentions | umls-concept:C0699900 | lld:lifeskim |
| pubmed-article:12046690 | lifeskim:mentions | umls-concept:C0243125 | lld:lifeskim |
| pubmed-article:12046690 | lifeskim:mentions | umls-concept:C1999216 | lld:lifeskim |
| pubmed-article:12046690 | lifeskim:mentions | umls-concept:C0332206 | lld:lifeskim |
| pubmed-article:12046690 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:12046690 | lifeskim:mentions | umls-concept:C0251991 | lld:lifeskim |
| pubmed-article:12046690 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:12046690 | pubmed:dateCreated | 2002-6-5 | lld:pubmed |
| pubmed-article:12046690 | pubmed:abstractText | The mechanism by which lipopolysaccharide (LPS) or phorbol 12-myristate 13-acetate (PMA) induces production of proinflammatory cytokines in murine macrophages, and the role of phosphatidylinositol 3-kinase (PI3-kinase) have not been well investigated. Activation of nuclear factor kappaB (NF-kappaB) is initiated by the phosphorylation of the inhibitory subunit, IkappaB, which targets IkappaB for degradation and leads to the release of active NF-kappaB. In this study we demonstrate that 2-(4-morpholinyl)-8-phenylchromone (LY294002), which inhibits PI3-kinase, specifically inhibited degradation of IkappaBalpha in RAW264.7 cells stimulated with interferon-gamma (IFN-gamma) plus LPS or IFN-gamma plus PMA. To elucidate the importance of this activity in RAW264.7 cells, we examined tumor necrosis factor-alpha (TNF-alpha) and interleukin (IL)-6 production in the activated cells. Pretreatment of the cells with LY294002 resulted in the inhibition of TNF-alpha and IL-6 production in RAW264.7 cells stimulated with IFN-gamma plus LPS or IFN-gamma plus PMA. Furthermore, LY294002 inhibited the production of nitric oxide (NO) in RAW264.7 cells stimulated with IFN-gamma plus LPS or IFN-gamma plus PMA. LY294002 also inhibited inducible NO synthase (iNOS) mRNA expression in the activated RAW264.7 cells. In conclusion, the present results suggest that PI3-kinase is involved in the signal transduction pathway responsible for LPS- or PMA-mediated TNF-alpha and IL-6 production, and that LY294002 inhibits NO generation through blocking the degradation of IkappaBalpha in activated RAW264.7 cells. | lld:pubmed |
| pubmed-article:12046690 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12046690 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12046690 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12046690 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12046690 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12046690 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12046690 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12046690 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12046690 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12046690 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12046690 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12046690 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12046690 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12046690 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12046690 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12046690 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12046690 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12046690 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12046690 | pubmed:issn | 0742-2091 | lld:pubmed |
| pubmed-article:12046690 | pubmed:author | pubmed-author:HSUF JFJ | lld:pubmed |
| pubmed-article:12046690 | pubmed:author | pubmed-author:LalK BKB | lld:pubmed |
| pubmed-article:12046690 | pubmed:author | pubmed-author:ParkS JSJ | lld:pubmed |
| pubmed-article:12046690 | pubmed:author | pubmed-author:HongS HSH | lld:pubmed |
| pubmed-article:12046690 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12046690 | pubmed:volume | 18 | lld:pubmed |
| pubmed-article:12046690 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12046690 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12046690 | pubmed:pagination | 121-30 | lld:pubmed |
| pubmed-article:12046690 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:12046690 | pubmed:meshHeading | pubmed-meshheading:12046690... | lld:pubmed |
| pubmed-article:12046690 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12046690 | pubmed:articleTitle | Degradation of IkappaBalpha in activated RAW264.7 cells is blocked by the phosphatidylinositol 3-kinase inhibitor LY294002. | lld:pubmed |
| pubmed-article:12046690 | pubmed:affiliation | Department of Oriental Pharmacy, Korea Institute of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Chonbuk. | lld:pubmed |
| pubmed-article:12046690 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12046690 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12046690 | lld:pubmed |
