| pubmed-article:12034816 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12034816 | lifeskim:mentions | umls-concept:C0004651 | lld:lifeskim |
| pubmed-article:12034816 | lifeskim:mentions | umls-concept:C1512977 | lld:lifeskim |
| pubmed-article:12034816 | lifeskim:mentions | umls-concept:C0073020 | lld:lifeskim |
| pubmed-article:12034816 | lifeskim:mentions | umls-concept:C0525021 | lld:lifeskim |
| pubmed-article:12034816 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:12034816 | lifeskim:mentions | umls-concept:C0332466 | lld:lifeskim |
| pubmed-article:12034816 | lifeskim:mentions | umls-concept:C0013682 | lld:lifeskim |
| pubmed-article:12034816 | lifeskim:mentions | umls-concept:C1707455 | lld:lifeskim |
| pubmed-article:12034816 | lifeskim:mentions | umls-concept:C2349975 | lld:lifeskim |
| pubmed-article:12034816 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:12034816 | pubmed:dateCreated | 2002-5-29 | lld:pubmed |
| pubmed-article:12034816 | pubmed:abstractText | The integrase of the phage PhiC31 recombines an attP site in the phage genome with a chromosomal attB site of its Streptomyces host. We have utilized the integrase-mediated reaction to achieve episomal and genomic deletion of a reporter gene in mammalian cells, and provide the first comparison of its efficiency with other recombinases in a new assay system. This assay demonstrated that the efficiency of PhiC31-integrase is significantly enhanced by the C-terminal, but not the N-terminal, addition of a nuclear localization signal and becomes comparable with that of the widely used Cre/loxP system. Furthermore, we found that the improved FLP recombinase, FLPe, exhibits only 10% recombination activity on chromosomal targets as compared with Cre, whereas the Anabaena derived XisA recombinase is essentially inactive in mammalian cells. These results provide the first demonstration that a nuclear localisation signal and its position within a recombinase can be important for its efficiency in mammalian cells and establish the improved PhiC31-integrase as a new tool for genome engineering. | lld:pubmed |
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| pubmed-article:12034816 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:12034816 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:12034816 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12034816 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:12034816 | pubmed:issn | 1362-4962 | lld:pubmed |
| pubmed-article:12034816 | pubmed:author | pubmed-author:KühnRalfR | lld:pubmed |
| pubmed-article:12034816 | pubmed:author | pubmed-author:AndreasSusann... | lld:pubmed |
| pubmed-article:12034816 | pubmed:author | pubmed-author:SchwenkFriede... | lld:pubmed |
| pubmed-article:12034816 | pubmed:author | pubmed-author:Küter-LuksBir... | lld:pubmed |
| pubmed-article:12034816 | pubmed:author | pubmed-author:FaustNicoleN | lld:pubmed |
| pubmed-article:12034816 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:12034816 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:12034816 | pubmed:volume | 30 | lld:pubmed |
| pubmed-article:12034816 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12034816 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12034816 | pubmed:pagination | 2299-306 | lld:pubmed |
| pubmed-article:12034816 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:12034816 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12034816 | pubmed:articleTitle | Enhanced efficiency through nuclear localization signal fusion on phage PhiC31-integrase: activity comparison with Cre and FLPe recombinase in mammalian cells. | lld:pubmed |
| pubmed-article:12034816 | pubmed:affiliation | Artemis Pharmaceuticals GmbH, Neurather Ring 1, 51063 Köln, Germany. | lld:pubmed |
| pubmed-article:12034816 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12034816 | pubmed:publicationType | Comparative Study | lld:pubmed |
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