| pubmed-article:12032154 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12032154 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:12032154 | lifeskim:mentions | umls-concept:C0085828 | lld:lifeskim |
| pubmed-article:12032154 | lifeskim:mentions | umls-concept:C0021641 | lld:lifeskim |
| pubmed-article:12032154 | lifeskim:mentions | umls-concept:C0037083 | lld:lifeskim |
| pubmed-article:12032154 | lifeskim:mentions | umls-concept:C0032214 | lld:lifeskim |
| pubmed-article:12032154 | lifeskim:mentions | umls-concept:C0086860 | lld:lifeskim |
| pubmed-article:12032154 | lifeskim:mentions | umls-concept:C1514562 | lld:lifeskim |
| pubmed-article:12032154 | lifeskim:mentions | umls-concept:C0162326 | lld:lifeskim |
| pubmed-article:12032154 | lifeskim:mentions | umls-concept:C1710082 | lld:lifeskim |
| pubmed-article:12032154 | lifeskim:mentions | umls-concept:C0065558 | lld:lifeskim |
| pubmed-article:12032154 | lifeskim:mentions | umls-concept:C0031586 | lld:lifeskim |
| pubmed-article:12032154 | lifeskim:mentions | umls-concept:C0013879 | lld:lifeskim |
| pubmed-article:12032154 | lifeskim:mentions | umls-concept:C1554080 | lld:lifeskim |
| pubmed-article:12032154 | lifeskim:mentions | umls-concept:C1706198 | lld:lifeskim |
| pubmed-article:12032154 | lifeskim:mentions | umls-concept:C0449255 | lld:lifeskim |
| pubmed-article:12032154 | lifeskim:mentions | umls-concept:C0962754 | lld:lifeskim |
| pubmed-article:12032154 | pubmed:issue | 31 | lld:pubmed |
| pubmed-article:12032154 | pubmed:dateCreated | 2002-7-29 | lld:pubmed |
| pubmed-article:12032154 | pubmed:abstractText | Insulin stimulates malic enzyme (ME)-chloramphenicol acetyltransferase (CAT) and collagenase-1-CAT fusion gene expression in H4IIE cells through identical activator protein-1 (AP-1) motifs. In contrast, insulin and phorbol esters only stimulate collagenase-1-CAT and not ME-CAT fusion gene expression in HeLa cells. The experiments in this article were designed to explore the molecular basis for this differential cell type- and gene-specific regulation. The results highlight the influence of three variables, namely promoter context, AP-1 flanking sequence, and accessory elements that modulate insulin and phorbol ester signaling through the AP-1 motif. Thus, fusion gene transfection and proteolytic clipping gel retardation assays suggest that the AP-1 flanking sequence affects the conformation of AP-1 binding to the collagenase-1 and ME AP-1 motifs such that it selectively binds the latter in a fully activated state. However, this influence of ME AP-1 flanking sequence is dependent on promoter context. Thus, the ME AP-1 motif will mediate both an insulin and phorbol ester response in HeLa cells when introduced into either the collagenase-1 promoter or a specific heterologous promoter. But even in the context of the collagenase-1 promoter, the effects of both insulin and phorbol esters, mediated through the ME AP-1 motif are dependent on accessory factors. | lld:pubmed |
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| pubmed-article:12032154 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:12032154 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:12032154 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12032154 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:12032154 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:12032154 | pubmed:author | pubmed-author:AyalaJulio... | lld:pubmed |
| pubmed-article:12032154 | pubmed:author | pubmed-author:StreeperRyan... | lld:pubmed |
| pubmed-article:12032154 | pubmed:author | pubmed-author:SvitekChristi... | lld:pubmed |
| pubmed-article:12032154 | pubmed:author | pubmed-author:GoldmanJoshua... | lld:pubmed |
| pubmed-article:12032154 | pubmed:author | pubmed-author:OeserJames... | lld:pubmed |
| pubmed-article:12032154 | pubmed:author | pubmed-author:O'BrienRichar... | lld:pubmed |
| pubmed-article:12032154 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12032154 | pubmed:day | 2 | lld:pubmed |
| pubmed-article:12032154 | pubmed:volume | 277 | lld:pubmed |
| pubmed-article:12032154 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12032154 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12032154 | pubmed:pagination | 27935-44 | lld:pubmed |
| pubmed-article:12032154 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:12032154 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12032154 | pubmed:articleTitle | Accessory elements, flanking DNA sequence, and promoter context play key roles in determining the efficacy of insulin and phorbol ester signaling through the malic enzyme and collagenase-1 AP-1 motifs. | lld:pubmed |
| pubmed-article:12032154 | pubmed:affiliation | Department of Molecular Physiology and Biophysics, Vanderbilt University Medical School, Nashville, Tennessee 37232, USA. | lld:pubmed |
| pubmed-article:12032154 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12032154 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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