| pubmed-article:12023344 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12023344 | lifeskim:mentions | umls-concept:C0024264 | lld:lifeskim |
| pubmed-article:12023344 | lifeskim:mentions | umls-concept:C0003320 | lld:lifeskim |
| pubmed-article:12023344 | lifeskim:mentions | umls-concept:C0003316 | lld:lifeskim |
| pubmed-article:12023344 | lifeskim:mentions | umls-concept:C0679199 | lld:lifeskim |
| pubmed-article:12023344 | lifeskim:mentions | umls-concept:C0439064 | lld:lifeskim |
| pubmed-article:12023344 | lifeskim:mentions | umls-concept:C1707689 | lld:lifeskim |
| pubmed-article:12023344 | lifeskim:mentions | umls-concept:C1705938 | lld:lifeskim |
| pubmed-article:12023344 | lifeskim:mentions | umls-concept:C1527178 | lld:lifeskim |
| pubmed-article:12023344 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:12023344 | pubmed:dateCreated | 2002-5-22 | lld:pubmed |
| pubmed-article:12023344 | pubmed:abstractText | Four HLA-DR-restricted HIV-derived Th lymphocyte (HTL) epitopes cross-reactive with the murine I-A(b) class II molecule were used to evaluate different vaccine design strategies to simultaneously induce multiple HTL responses. All four epitopes were immunogenic in H-2(b) mice, demonstrating the feasibility of murine models to evaluate epitope-based vaccines destined for human use. Immunization with a pool of peptides induced responses against all four epitopes; illustrating immunodominance does not prevent the induction of balanced multispecific responses. When different delivery systems were evaluated, a multiple Ag peptide construct was found to be less efficient than a linear polypeptide encompassing all four epitopes. Further characterization of linear polypeptide revealed that the sequential arrangement of the epitopes created a junctional epitope with high affinity class II binding. Disruption of this junctional epitope through the introduction of a GPGPG spacer restored the immunogenicity against all four epitopes. Finally, we demonstrate that a GPGPG spacer construct can be used to induce HTL responses by either polypeptide or DNA immunization, highlighting the flexibility of the approach. | lld:pubmed |
| pubmed-article:12023344 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12023344 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12023344 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12023344 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:12023344 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:12023344 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12023344 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12023344 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:12023344 | pubmed:issn | 0022-1767 | lld:pubmed |
| pubmed-article:12023344 | pubmed:author | pubmed-author:HigashimotoYu... | lld:pubmed |
| pubmed-article:12023344 | pubmed:author | pubmed-author:AppellaEttore... | lld:pubmed |
| pubmed-article:12023344 | pubmed:author | pubmed-author:SetteAlessand... | lld:pubmed |
| pubmed-article:12023344 | pubmed:author | pubmed-author:NewmanMarkM | lld:pubmed |
| pubmed-article:12023344 | pubmed:author | pubmed-author:LivingstonBri... | lld:pubmed |
| pubmed-article:12023344 | pubmed:author | pubmed-author:CrimiClaireC | lld:pubmed |
| pubmed-article:12023344 | pubmed:author | pubmed-author:SidneyJohnJ | lld:pubmed |
| pubmed-article:12023344 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12023344 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:12023344 | pubmed:volume | 168 | lld:pubmed |
| pubmed-article:12023344 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12023344 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12023344 | pubmed:pagination | 5499-506 | lld:pubmed |
| pubmed-article:12023344 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:12023344 | pubmed:meshHeading | pubmed-meshheading:12023344... | lld:pubmed |
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| pubmed-article:12023344 | pubmed:meshHeading | pubmed-meshheading:12023344... | lld:pubmed |
| pubmed-article:12023344 | pubmed:meshHeading | pubmed-meshheading:12023344... | lld:pubmed |
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| pubmed-article:12023344 | pubmed:meshHeading | pubmed-meshheading:12023344... | lld:pubmed |
| pubmed-article:12023344 | pubmed:meshHeading | pubmed-meshheading:12023344... | lld:pubmed |
| pubmed-article:12023344 | pubmed:meshHeading | pubmed-meshheading:12023344... | lld:pubmed |
| pubmed-article:12023344 | pubmed:meshHeading | pubmed-meshheading:12023344... | lld:pubmed |
| pubmed-article:12023344 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12023344 | pubmed:articleTitle | A rational strategy to design multiepitope immunogens based on multiple Th lymphocyte epitopes. | lld:pubmed |
| pubmed-article:12023344 | pubmed:affiliation | Epimmune, San Diego, CA 92121, USA. blivingston@epimmune.com | lld:pubmed |
| pubmed-article:12023344 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12023344 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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