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pubmed-article:12023086pubmed:abstractTextAgrobacterium tumefaciens T pili are long semi-rigid, flexuous filaments of 10 nm diameter that are primarily composed of T pilin cyclized protein subunits. The cyclic character of T pilin apparently confers a high level of structural stability on the T pilus. Purified T pili subjected to extreme environmental conditions such as acid and alkali, including glycerol remained relatively unaffected morphologically. T pili lost their semi-rigidity when subjected to high temperatures and high pH, and dissociated into donut shaped subunits when exposed to Triton X-100. Sodium dodecyl sulfate increased the uptake of uranyl acetate exposing a 2 nm wide lumen running the length of the T pilus filament.lld:pubmed
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pubmed-article:12023086pubmed:dateRevised2011-11-17lld:pubmed
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pubmed-article:12023086pubmed:articleTitleThe Agrobacterium tumefaciens T pilus composed of cyclic T pilin is highly resilient to extreme environments.lld:pubmed
pubmed-article:12023086pubmed:affiliationDavis Crown Gall Group, University of California, One Shields Avenue, Davis 95616, USA.lld:pubmed
pubmed-article:12023086pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:12023086pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
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