| pubmed-article:11983898 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11983898 | lifeskim:mentions | umls-concept:C0205127 | lld:lifeskim |
| pubmed-article:11983898 | lifeskim:mentions | umls-concept:C0205093 | lld:lifeskim |
| pubmed-article:11983898 | lifeskim:mentions | umls-concept:C0026046 | lld:lifeskim |
| pubmed-article:11983898 | lifeskim:mentions | umls-concept:C0750572 | lld:lifeskim |
| pubmed-article:11983898 | lifeskim:mentions | umls-concept:C1442080 | lld:lifeskim |
| pubmed-article:11983898 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:11983898 | pubmed:dateCreated | 2002-5-1 | lld:pubmed |
| pubmed-article:11983898 | pubmed:abstractText | We developed a stochastic model of microtubule (MT) assembly dynamics that estimates tubulin-tubulin bond energies, mechanical energy stored in the lattice dimers, and the size of the tubulin-GTP cap at MT tips. First, a simple assembly/disassembly state model was used to screen possible combinations of lateral bond energy (DeltaG(Lat)) and longitudinal bond energy (DeltaG(Long)) plus the free energy of immobilizing a dimer in the MT lattice (DeltaG(S)) for rates of MT growth and shortening measured experimentally. This analysis predicts DeltaG(Lat) in the range of -3.2 to -5.7 k(B)T and DeltaG(Long) plus DeltaG(S) in the range of -6.8 to -9.4 k(B)T. Based on these estimates, the energy of conformational stress for a single tubulin-GDP dimer in the lattice is 2.1-2.5 k(B)T. Second, we studied how tubulin-GTP cap size fluctuates with different hydrolysis rules and show that a mechanism of directly coupling subunit addition to hydrolysis fails to support MT growth, whereas a finite hydrolysis rate allows growth. By adding rules to mimic the mechanical constraints present at the MT tip, the model generates tubulin-GTP caps similar in size to experimental estimates. Finally, by combining assembly/disassembly and cap dynamics, we generate MT dynamic instability with rates and transition frequencies similar to those measured experimentally. Our model serves as a platform to examine GTP-cap dynamics and allows predictions of how MT-associated proteins and other effectors alter the energetics of MT assembly. | lld:pubmed |
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| pubmed-article:11983898 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11983898 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11983898 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:11983898 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11983898 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:11983898 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:11983898 | pubmed:author | pubmed-author:CassimerisLyn... | lld:pubmed |
| pubmed-article:11983898 | pubmed:author | pubmed-author:VanBurenVince... | lld:pubmed |
| pubmed-article:11983898 | pubmed:author | pubmed-author:OddeDavid JDJ | lld:pubmed |
| pubmed-article:11983898 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11983898 | pubmed:day | 30 | lld:pubmed |
| pubmed-article:11983898 | pubmed:volume | 99 | lld:pubmed |
| pubmed-article:11983898 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11983898 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11983898 | pubmed:pagination | 6035-40 | lld:pubmed |
| pubmed-article:11983898 | pubmed:dateRevised | 2010-9-14 | lld:pubmed |
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| pubmed-article:11983898 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:11983898 | pubmed:articleTitle | Estimates of lateral and longitudinal bond energies within the microtubule lattice. | lld:pubmed |
| pubmed-article:11983898 | pubmed:affiliation | Department of Biological Sciences, Lehigh University, 111 Research Drive, Bethlehem, PA 18015, USA. | lld:pubmed |
| pubmed-article:11983898 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11983898 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:11983898 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:11983898 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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