| pubmed-article:11929690 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11929690 | lifeskim:mentions | umls-concept:C0003320 | lld:lifeskim |
| pubmed-article:11929690 | lifeskim:mentions | umls-concept:C0030956 | lld:lifeskim |
| pubmed-article:11929690 | lifeskim:mentions | umls-concept:C0204727 | lld:lifeskim |
| pubmed-article:11929690 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:11929690 | lifeskim:mentions | umls-concept:C0041221 | lld:lifeskim |
| pubmed-article:11929690 | lifeskim:mentions | umls-concept:C0598888 | lld:lifeskim |
| pubmed-article:11929690 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:11929690 | pubmed:dateCreated | 2002-4-3 | lld:pubmed |
| pubmed-article:11929690 | pubmed:abstractText | Trypomastigote forms of Trypanosoma cruzi excrete-secrete several molecules, which are immunodominant during the human infection. This complex antigenic mixture termed TESA (Trypomastigote Excreted-Secreted Antigens) presents a 150-160 kDa band that shows excellent specificity and sensitivity in Chagas' disease diagnosis by immunoblotting. Here we describe the isolation and the antigenic characterization of a recombinant peptide (TESA-1) containing a 10 kDa T. cruzi peptide that belongs to the 150-160 kDa TESA fraction. The clone was isolated by screening a T. cruzi genomic expression library with chagasic antibodies reactive to the 150-160 kDa band of TESA immunoblots. After expression, the recombinant peptide TESA-1 was purified and used to immunize rabbits. Anti-TESA-1 immunesera specifically recognized the 150-160 kDa fraction of TESA-blots from eight different T. cruzi strains. The TESA-1 peptide reacted with 82.2% of chagasic patient sera by immunoblotting, showing that it harbors most of the antigenic epitopes that account for the high reactivity of the 150-160 kDa band of TESA. | lld:pubmed |
| pubmed-article:11929690 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11929690 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11929690 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11929690 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11929690 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11929690 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11929690 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11929690 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11929690 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:11929690 | pubmed:issn | 0732-8893 | lld:pubmed |
| pubmed-article:11929690 | pubmed:author | pubmed-author:MatsumotoToki... | lld:pubmed |
| pubmed-article:11929690 | pubmed:author | pubmed-author:CotrimPaulo... | lld:pubmed |
| pubmed-article:11929690 | pubmed:author | pubmed-author:da... | lld:pubmed |
| pubmed-article:11929690 | pubmed:author | pubmed-author:StolfAnna... | lld:pubmed |
| pubmed-article:11929690 | pubmed:author | pubmed-author:UmezawaEufros... | lld:pubmed |
| pubmed-article:11929690 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11929690 | pubmed:volume | 42 | lld:pubmed |
| pubmed-article:11929690 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11929690 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11929690 | pubmed:pagination | 187-92 | lld:pubmed |
| pubmed-article:11929690 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:11929690 | pubmed:meshHeading | pubmed-meshheading:11929690... | lld:pubmed |
| pubmed-article:11929690 | pubmed:meshHeading | pubmed-meshheading:11929690... | lld:pubmed |
| pubmed-article:11929690 | pubmed:meshHeading | pubmed-meshheading:11929690... | lld:pubmed |
| pubmed-article:11929690 | pubmed:meshHeading | pubmed-meshheading:11929690... | lld:pubmed |
| pubmed-article:11929690 | pubmed:meshHeading | pubmed-meshheading:11929690... | lld:pubmed |
| pubmed-article:11929690 | pubmed:meshHeading | pubmed-meshheading:11929690... | lld:pubmed |
| pubmed-article:11929690 | pubmed:meshHeading | pubmed-meshheading:11929690... | lld:pubmed |
| pubmed-article:11929690 | pubmed:meshHeading | pubmed-meshheading:11929690... | lld:pubmed |
| pubmed-article:11929690 | pubmed:meshHeading | pubmed-meshheading:11929690... | lld:pubmed |
| pubmed-article:11929690 | pubmed:meshHeading | pubmed-meshheading:11929690... | lld:pubmed |
| pubmed-article:11929690 | pubmed:meshHeading | pubmed-meshheading:11929690... | lld:pubmed |
| pubmed-article:11929690 | pubmed:meshHeading | pubmed-meshheading:11929690... | lld:pubmed |
| pubmed-article:11929690 | pubmed:meshHeading | pubmed-meshheading:11929690... | lld:pubmed |
| pubmed-article:11929690 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:11929690 | pubmed:articleTitle | Trypanosoma cruzi: isolation of an immunodominant peptide of TESA (Trypomastigote Excreted-Secreted Antigens) by gene cloning. | lld:pubmed |
| pubmed-article:11929690 | pubmed:affiliation | Instituto Adolfo Lutz-São Paulo, Brazil. | lld:pubmed |
| pubmed-article:11929690 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11929690 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11929690 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11929690 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11929690 | lld:pubmed |
