| pubmed-article:11891583 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11891583 | lifeskim:mentions | umls-concept:C0034493 | lld:lifeskim |
| pubmed-article:11891583 | lifeskim:mentions | umls-concept:C0242692 | lld:lifeskim |
| pubmed-article:11891583 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:11891583 | lifeskim:mentions | umls-concept:C1159690 | lld:lifeskim |
| pubmed-article:11891583 | lifeskim:mentions | umls-concept:C0740230 | lld:lifeskim |
| pubmed-article:11891583 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:11891583 | pubmed:dateCreated | 2002-3-13 | lld:pubmed |
| pubmed-article:11891583 | pubmed:abstractText | Triads and transverse tubules isolated from mammalian skeletal muscle actively accumulated Na+ in the presence of K+ and Mg-ATP. Active Na+ transport exhibited a fast single-exponential phase, lasting 2 min, followed by slower linear uptake that continued for 10 minutes. Valinomycin stimulated Na+ uptake, suggesting it decreased a pump-generated membrane potential gradient (Vm) that prevented further Na+ accumulation. At the end of the fast uptake phase transverse tubule vesicles incubated in 30 mM external [Na+] attained a ratio [Na+]in/[Na+]out=13.4. From this ratio and the transverse tubule volume of 0.35 microl/mg protein measured in this work, [Na+]in=400 mM was calculated. Determinations of active K+ transport in triads, using 86Rb+ as tracer, showed a 30% decrease in vesicular 86Rb+ content two minutes after initiating the reaction, followed by a slower uptake phase during which vesicles regained their initial 86Rb+ content after 10 minutes. Transverse tubule volume increase during active Na+ transport-as shown by light scattering changes of isolated vesicles--presumably accounted for the secondary Na+ and 86Rb+ uptake phases. These combined results indicate that isolated triads have highly sealed transverse tubules that can be polarized effectively by the Na+ pump through the generation of significant Na+ gradients. | lld:pubmed |
| pubmed-article:11891583 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11891583 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11891583 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11891583 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11891583 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11891583 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11891583 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11891583 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11891583 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11891583 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:11891583 | pubmed:issn | 0022-2631 | lld:pubmed |
| pubmed-article:11891583 | pubmed:author | pubmed-author:HidalgoCC | lld:pubmed |
| pubmed-article:11891583 | pubmed:author | pubmed-author:DonosoPP | lld:pubmed |
| pubmed-article:11891583 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11891583 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:11891583 | pubmed:volume | 185 | lld:pubmed |
| pubmed-article:11891583 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11891583 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11891583 | pubmed:pagination | 257-63 | lld:pubmed |
| pubmed-article:11891583 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:11891583 | pubmed:meshHeading | pubmed-meshheading:11891583... | lld:pubmed |
| pubmed-article:11891583 | pubmed:meshHeading | pubmed-meshheading:11891583... | lld:pubmed |
| pubmed-article:11891583 | pubmed:meshHeading | pubmed-meshheading:11891583... | lld:pubmed |
| pubmed-article:11891583 | pubmed:meshHeading | pubmed-meshheading:11891583... | lld:pubmed |
| pubmed-article:11891583 | pubmed:meshHeading | pubmed-meshheading:11891583... | lld:pubmed |
| pubmed-article:11891583 | pubmed:meshHeading | pubmed-meshheading:11891583... | lld:pubmed |
| pubmed-article:11891583 | pubmed:meshHeading | pubmed-meshheading:11891583... | lld:pubmed |
| pubmed-article:11891583 | pubmed:meshHeading | pubmed-meshheading:11891583... | lld:pubmed |
| pubmed-article:11891583 | pubmed:meshHeading | pubmed-meshheading:11891583... | lld:pubmed |
| pubmed-article:11891583 | pubmed:meshHeading | pubmed-meshheading:11891583... | lld:pubmed |
| pubmed-article:11891583 | pubmed:meshHeading | pubmed-meshheading:11891583... | lld:pubmed |
| pubmed-article:11891583 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:11891583 | pubmed:articleTitle | Sodium transport in triads isolated from rabbit skeletal muscle. | lld:pubmed |
| pubmed-article:11891583 | pubmed:affiliation | Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Casilla 70005, Santiago 7, Chile. pdonoso@machi.med.uchile.cl | lld:pubmed |
| pubmed-article:11891583 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11891583 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
