| pubmed-article:11880538 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11880538 | lifeskim:mentions | umls-concept:C1522021 | lld:lifeskim |
| pubmed-article:11880538 | lifeskim:mentions | umls-concept:C0524869 | lld:lifeskim |
| pubmed-article:11880538 | lifeskim:mentions | umls-concept:C0596988 | lld:lifeskim |
| pubmed-article:11880538 | lifeskim:mentions | umls-concept:C1336633 | lld:lifeskim |
| pubmed-article:11880538 | lifeskim:mentions | umls-concept:C0883208 | lld:lifeskim |
| pubmed-article:11880538 | lifeskim:mentions | umls-concept:C0015111 | lld:lifeskim |
| pubmed-article:11880538 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:11880538 | lifeskim:mentions | umls-concept:C0439603 | lld:lifeskim |
| pubmed-article:11880538 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:11880538 | pubmed:dateCreated | 2002-3-6 | lld:pubmed |
| pubmed-article:11880538 | pubmed:abstractText | N-ethyl-N-nitrosourea (ENU) is a potent monofunctional ethylating agent that has been found to be mutagenic in a wide variety of organisms from viruses to mammalian germ cells. To elucidate the mutagenicity of ENU at the Tk(+/-) locus of mouse lymphoma cells and to confirm the ability of the mouse lymphoma assay (MLA) to detect both point mutations and large DNA alterations, Tk(+/-) L5178Y cells were exposed to different doses of ENU. Treatment of the cells with ENU resulted in a linear dose response with mutant frequencies of up to 16-fold over control. Evaluation of mutant clone size showed that 36% of the 100 microg/ml ENU-induced clones (66% in control) were small colony mutants and 64% (34% in control) were large colony mutants. DNA isolated from mutants in the control culture and the 100 microg/ml ENU treatment group was analyzed for loss of heterozygosity (LOH) using allele-specific PCR. The majority of the small colony mutants, both ENU-treated (97%) and spontaneous (91%), lost the Tk1b allele. The percentage of allele loss in ENU-induced large colony mutants was distinctly different from that of the control. Twenty-three percent of ENU-induced large colony mutants lost their Tk1b alleles, whereas 73% of the large colony mutants from the control culture lost the allele (P < 0.001). Overall, 50% of the Tk mutants from the 100 microg/ml ENU-treated cultures (86% in control) showed LOH. Our data indicate that ENU is a potent mutagen in mouse lymphoma cells and that 100 microg/ml ENU induces equal numbers of point mutations and chromosomal mutations. This study serves to verify that the MLA detects both point mutations and chromosomal mutations. | lld:pubmed |
| pubmed-article:11880538 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11880538 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11880538 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11880538 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11880538 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11880538 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11880538 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11880538 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:11880538 | pubmed:issn | 0267-8357 | lld:pubmed |
| pubmed-article:11880538 | pubmed:author | pubmed-author:ChenTaoT | lld:pubmed |
| pubmed-article:11880538 | pubmed:author | pubmed-author:Harrington-Br... | lld:pubmed |
| pubmed-article:11880538 | pubmed:author | pubmed-author:MooreMartha... | lld:pubmed |
| pubmed-article:11880538 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11880538 | pubmed:volume | 17 | lld:pubmed |
| pubmed-article:11880538 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11880538 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11880538 | pubmed:pagination | 105-9 | lld:pubmed |
| pubmed-article:11880538 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:11880538 | pubmed:meshHeading | pubmed-meshheading:11880538... | lld:pubmed |
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| pubmed-article:11880538 | pubmed:meshHeading | pubmed-meshheading:11880538... | lld:pubmed |
| pubmed-article:11880538 | pubmed:meshHeading | pubmed-meshheading:11880538... | lld:pubmed |
| pubmed-article:11880538 | pubmed:meshHeading | pubmed-meshheading:11880538... | lld:pubmed |
| pubmed-article:11880538 | pubmed:meshHeading | pubmed-meshheading:11880538... | lld:pubmed |
| pubmed-article:11880538 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:11880538 | pubmed:articleTitle | Mutant frequencies and loss of heterozygosity induced by N-ethyl-N-nitrosourea in the thymidine kinase gene of L5178Y/TK(+/-)-3.7.2C mouse lymphoma cells. | lld:pubmed |
| pubmed-article:11880538 | pubmed:affiliation | Division of Genetic and Reproductive Toxicology, National Center for Toxicological Research, FDA, Jefferson, AR 72079, USA. tchen@nctr.fda.gov | lld:pubmed |
| pubmed-article:11880538 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11880538 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:11880538 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:21877 | entrezgene:pubmed | pubmed-article:11880538 | lld:entrezgene |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11880538 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11880538 | lld:pubmed |
