| pubmed-article:11857474 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11857474 | lifeskim:mentions | umls-concept:C0085979 | lld:lifeskim |
| pubmed-article:11857474 | lifeskim:mentions | umls-concept:C0037868 | lld:lifeskim |
| pubmed-article:11857474 | lifeskim:mentions | umls-concept:C0003250 | lld:lifeskim |
| pubmed-article:11857474 | lifeskim:mentions | umls-concept:C1179841 | lld:lifeskim |
| pubmed-article:11857474 | lifeskim:mentions | umls-concept:C0204727 | lld:lifeskim |
| pubmed-article:11857474 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:11857474 | lifeskim:mentions | umls-concept:C1539063 | lld:lifeskim |
| pubmed-article:11857474 | lifeskim:mentions | umls-concept:C1264633 | lld:lifeskim |
| pubmed-article:11857474 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:11857474 | pubmed:dateCreated | 2002-2-21 | lld:pubmed |
| pubmed-article:11857474 | pubmed:abstractText | Membrane fragments were obtained from guinea pig spermatozoa by mechanical shearing. A membrane-enriched fraction was separated from other cellular debris, mainly sperm nuclei and tails, by centrifugation on 20% Ficoll 70 solution. Peri-acrosomal plasma membrane protein, D40, enriched fraction was separated from this membrane preparation using a mouse monoclonal antibody to D40 attached to magnetic beads. Enrichment of D40 antigen in this fraction was demonstrated by western blotting. The method provides a preparative route to a membrane, the constituents of which play an important role in sperm recognition of the zona pellucida and the acrosome reaction. Some constituents of the peri-acrosomal plasma membrane over the equatorial segment of the acrosome may also play a role in sperm docking with the oocyte plasma membrane and fusion of the two cells. | lld:pubmed |
| pubmed-article:11857474 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11857474 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11857474 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11857474 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11857474 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11857474 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11857474 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11857474 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11857474 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:11857474 | pubmed:issn | 0022-104X | lld:pubmed |
| pubmed-article:11857474 | pubmed:author | pubmed-author:IlayperumaII | lld:pubmed |
| pubmed-article:11857474 | pubmed:copyrightInfo | Copyright 2002 Wiley-Liss, Inc. | lld:pubmed |
| pubmed-article:11857474 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11857474 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:11857474 | pubmed:volume | 292 | lld:pubmed |
| pubmed-article:11857474 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11857474 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11857474 | pubmed:pagination | 402-10 | lld:pubmed |
| pubmed-article:11857474 | pubmed:dateRevised | 2010-6-2 | lld:pubmed |
| pubmed-article:11857474 | pubmed:meshHeading | pubmed-meshheading:11857474... | lld:pubmed |
| pubmed-article:11857474 | pubmed:meshHeading | pubmed-meshheading:11857474... | lld:pubmed |
| pubmed-article:11857474 | pubmed:meshHeading | pubmed-meshheading:11857474... | lld:pubmed |
| pubmed-article:11857474 | pubmed:meshHeading | pubmed-meshheading:11857474... | lld:pubmed |
| pubmed-article:11857474 | pubmed:meshHeading | pubmed-meshheading:11857474... | lld:pubmed |
| pubmed-article:11857474 | pubmed:meshHeading | pubmed-meshheading:11857474... | lld:pubmed |
| pubmed-article:11857474 | pubmed:meshHeading | pubmed-meshheading:11857474... | lld:pubmed |
| pubmed-article:11857474 | pubmed:meshHeading | pubmed-meshheading:11857474... | lld:pubmed |
| pubmed-article:11857474 | pubmed:meshHeading | pubmed-meshheading:11857474... | lld:pubmed |
| pubmed-article:11857474 | pubmed:meshHeading | pubmed-meshheading:11857474... | lld:pubmed |
| pubmed-article:11857474 | pubmed:meshHeading | pubmed-meshheading:11857474... | lld:pubmed |
| pubmed-article:11857474 | pubmed:meshHeading | pubmed-meshheading:11857474... | lld:pubmed |
| pubmed-article:11857474 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:11857474 | pubmed:articleTitle | Isolation of the peri-acrosomal plasma membrane protein D40 enriched fraction from guinea pig spermatozoa using a monoclonal antibody. | lld:pubmed |
| pubmed-article:11857474 | pubmed:affiliation | Department of Anatomy and Structural Biology, School of Medical Sciences, University of Otago, Dunedin, New Zealand. isurani@anato.ruh.ac.lk | lld:pubmed |
| pubmed-article:11857474 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11857474 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
