| pubmed-article:11850499 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11850499 | lifeskim:mentions | umls-concept:C0999699 | lld:lifeskim |
| pubmed-article:11850499 | lifeskim:mentions | umls-concept:C0596981 | lld:lifeskim |
| pubmed-article:11850499 | lifeskim:mentions | umls-concept:C0205145 | lld:lifeskim |
| pubmed-article:11850499 | lifeskim:mentions | umls-concept:C0028351 | lld:lifeskim |
| pubmed-article:11850499 | lifeskim:mentions | umls-concept:C0025465 | lld:lifeskim |
| pubmed-article:11850499 | lifeskim:mentions | umls-concept:C0178719 | lld:lifeskim |
| pubmed-article:11850499 | lifeskim:mentions | umls-concept:C0600437 | lld:lifeskim |
| pubmed-article:11850499 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:11850499 | lifeskim:mentions | umls-concept:C0030685 | lld:lifeskim |
| pubmed-article:11850499 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:11850499 | lifeskim:mentions | umls-concept:C0680255 | lld:lifeskim |
| pubmed-article:11850499 | lifeskim:mentions | umls-concept:C0391871 | lld:lifeskim |
| pubmed-article:11850499 | lifeskim:mentions | umls-concept:C1283071 | lld:lifeskim |
| pubmed-article:11850499 | lifeskim:mentions | umls-concept:C1963578 | lld:lifeskim |
| pubmed-article:11850499 | lifeskim:mentions | umls-concept:C2348867 | lld:lifeskim |
| pubmed-article:11850499 | pubmed:issue | Pt 1 | lld:pubmed |
| pubmed-article:11850499 | pubmed:dateCreated | 2002-2-18 | lld:pubmed |
| pubmed-article:11850499 | pubmed:abstractText | In smooth muscle the spontaneous Ca2+ release from the sarcoplasmic reticulum (SR) occurs at preferred locations called frequent discharge sites (FDSs) giving rise to localized intracellular Ca2+ transients (Ca2+ sparks). Laser scanning confocal microscopy of fluo-3-loaded single myocytes freshly isolated from small mesenteric arteries of guinea-pig was used to investigate the action of nitric oxide (NO) donors and noradrenaline on the position and activity of FDSs and on global intracellular Ca2+ concentration ([Ca2+]i). In 8 % of cells 'microsparks', Ca2+ release events smaller in duration, spread and amplitude than Ca2+ sparks were observed. The location of the initiation point of Ca2+ sparks observed during line-scan imaging was found to 'jitter' by +/- 0.41 microm. However, the general position of an FDS within the cell did not change; most FDSs were close (within 1.2 +/- 0.1 microm) to the cell membrane and often multiple FDSs occurred in one confocal plane of the cell. In the resting state, NO donors S-nitroso-N-acetylpenicillamine (SNAP; 50 microM) and sodium nitroprusside (SNP; 100 microM) did not change the general position of FDSs and slightly depressed their activity, but did not affect the global [Ca2+]i significantly. Application of noradrenaline (1-10 microM) increased Ca2+ spark frequency at existing FDS(s) leading to a Ca2+ wave. The increase in FDS activity and in global [Ca2+]i produced by noradrenaline were inhibited by the presence of SNAP or SNP but not by 8-bromoguanosine cyclic 3',5'-monophosphate (8-Br-cGMP; 100 microM). In the presence of 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), inhibitor of soluble guanylate cyclase, SNAP and SNP still exerted their effects on the noradrenaline response. These results suggest that SNAP and SNP inhibit the noradrenaline-evoked rise in global [Ca2+]i by a cGMP-independent mechanism and that part of this effect is due to inhibition of the activity of FDSs; moreover, only the activity, but not the position, of FDSs is changed by either stimulant or inhibitory substances. | lld:pubmed |
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| pubmed-article:11850499 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11850499 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11850499 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11850499 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:11850499 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11850499 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:11850499 | pubmed:issn | 0022-3751 | lld:pubmed |
| pubmed-article:11850499 | pubmed:author | pubmed-author:GordienkoDmit... | lld:pubmed |
| pubmed-article:11850499 | pubmed:author | pubmed-author:PucovskýVladi... | lld:pubmed |
| pubmed-article:11850499 | pubmed:author | pubmed-author:BoltonThomas... | lld:pubmed |
| pubmed-article:11850499 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11850499 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:11850499 | pubmed:volume | 539 | lld:pubmed |
| pubmed-article:11850499 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11850499 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11850499 | pubmed:pagination | 25-39 | lld:pubmed |
| pubmed-article:11850499 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:11850499 | pubmed:meshHeading | pubmed-meshheading:11850499... | lld:pubmed |
| pubmed-article:11850499 | pubmed:meshHeading | pubmed-meshheading:11850499... | lld:pubmed |
| pubmed-article:11850499 | pubmed:meshHeading | pubmed-meshheading:11850499... | lld:pubmed |
