Linked Life Data

11827177

Source:http://linkedlifedata.com/resource/pubmed/id/11827177

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pubmed-article:11827177pubmed:abstractTextThe protein kinase CK2 is composed of two catalytic alpha- or alpha'- and two regulatory beta-subunits. In mammalian cells there is ample evidence for the presence of individual CK2 subunits beside the holoenzyme. By immunofluorescence studies using peptide antibodies which allow us to detect the CK2alpha-, alpha'- and beta-subunits we found all three subunits to be co-localized with a 58 KDa Golgi protein which is specific for the Golgi complex. Subfractionation studies using dog pancreas cells revealed the presence of all three subunits of CK2 at the smooth endoplasmic reticulum (sER)/Golgi fraction whereas the rough endoplasmic reticulum (rER) harboured only the catalytic alpha- and alpha'-subunits. We found that the microsomal preparation from dog pancreas cells contained CK2 which phosphorylated a CK2 specific synthetic peptide and which was heparin sensitive. Furthermore, we could immunoprecipitate the CK2alpha-subunit that exhibited a kinase activity which phosphorylated a CK2 specific substrate and which was heparin sensitive. Protease digestion experiments revealed that the CK2 subunits were located on the cytosolic side of the rER and the sER/Golgi complex. Thus, we could demonstrate an asymmetric distribution of the CK2 subunits at the rER and sER/Golgi complex. Since the CK2alpha- and alpha'-subunits exhibit a substrate specificity which is different from the CK2 holoenzyme one might speculate that the asymmetric distribution of the CK2 holoenzyme and the CK2 catalytic subunits may have regulatory functions.lld:pubmed
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pubmed-article:11827177pubmed:articleTitleLocalization of individual subunits of protein kinase CK2 to the endoplasmic reticulum and to the Golgi apparatus.lld:pubmed
pubmed-article:11827177pubmed:affiliationMedical Biochemistry and Molecular Biology, University of the Saarland, Homburg/Saar, Germany.lld:pubmed
pubmed-article:11827177pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:11827177pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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