| pubmed-article:11760086 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11760086 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:11760086 | lifeskim:mentions | umls-concept:C0012984 | lld:lifeskim |
| pubmed-article:11760086 | lifeskim:mentions | umls-concept:C0018951 | lld:lifeskim |
| pubmed-article:11760086 | lifeskim:mentions | umls-concept:C0376152 | lld:lifeskim |
| pubmed-article:11760086 | lifeskim:mentions | umls-concept:C1332710 | lld:lifeskim |
| pubmed-article:11760086 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:11760086 | lifeskim:mentions | umls-concept:C0443252 | lld:lifeskim |
| pubmed-article:11760086 | lifeskim:mentions | umls-concept:C1514926 | lld:lifeskim |
| pubmed-article:11760086 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:11760086 | pubmed:dateCreated | 2001-12-10 | lld:pubmed |
| pubmed-article:11760086 | pubmed:abstractText | Human CD34+ cells have been shown to retain long-term hematopoietic engrafting potential in preclinical and clinical studies. However, recent studies of human and murine CD34- stem cells suggest that these are functionally important early progenitors. Using autologous transplantation, we investigated whether canine CD34 and CD34- marrow cells could be transduced and give rise to long-term hematopoiesis. CD34+Lin- and CD34-Lin- cell populations purified by fluorescence-activated cell sorting were separately cocultivated with retroviral vectors LN (CD34+Lin-) and LNY (CD34-Lin-), which carry the neomycin (neo) gene. After myeloablative total body irradiation (920 cGy), 3 dogs received transplants of both CD34+Lin- cells and CD34-Lin- cells and 2 dogs received only CD34-Lin- cells. Untransduced autologous marrow cells were given to ensure hematopoietic recovery. Using CFU-C assays, transduction efficiencies of CD34+Lin- cells ranged from 6% to 18% with no CFU-C formation from CD34-Lin- cells. PCR-based detection of the neo gene from WBCs was used to detect transduced cells weekly after transplantation. Additional PCR studies in 3 dogs given both CD34+Lin- and CD34-Lin- cells were performed on monocytes, granulocytes, and T cells (2 dogs, one at 7.5 months and the other at 9 months) and granulocytes (1 dog at 12 months). LN was detected up to 12 months posttransplantation in WBCs and mono-myeloid and lymphoid populations from 3 dogs receiving transplants of transduced CD34+Lin- cells. LNY was not detected at any time after transplantation in 5 dogs that received transduced CD34-Lin- cells. Whereas canine CD34+Lin- marrow cells contributed to long-term multilineage hematopoiesis, progeny of CD34-Lin- progenitor cells were not detected after transplantation in these experiments. | lld:pubmed |
| pubmed-article:11760086 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11760086 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11760086 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11760086 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11760086 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11760086 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11760086 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11760086 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11760086 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11760086 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11760086 | pubmed:issn | 1083-8791 | lld:pubmed |
| pubmed-article:11760086 | pubmed:author | pubmed-author:StorbRR | lld:pubmed |
| pubmed-article:11760086 | pubmed:author | pubmed-author:NashR ARA | lld:pubmed |
| pubmed-article:11760086 | pubmed:author | pubmed-author:KingJ WJWJr | lld:pubmed |
| pubmed-article:11760086 | pubmed:author | pubmed-author:BruniDD | lld:pubmed |
| pubmed-article:11760086 | pubmed:author | pubmed-author:McSweeneyP... | lld:pubmed |
| pubmed-article:11760086 | pubmed:author | pubmed-author:GoernerM AMA | lld:pubmed |
| pubmed-article:11760086 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11760086 | pubmed:volume | 7 | lld:pubmed |
| pubmed-article:11760086 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11760086 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11760086 | pubmed:pagination | 543-51 | lld:pubmed |
| pubmed-article:11760086 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:11760086 | pubmed:meshHeading | pubmed-meshheading:11760086... | lld:pubmed |
| pubmed-article:11760086 | pubmed:meshHeading | pubmed-meshheading:11760086... | lld:pubmed |
| pubmed-article:11760086 | pubmed:meshHeading | pubmed-meshheading:11760086... | lld:pubmed |
| pubmed-article:11760086 | pubmed:meshHeading | pubmed-meshheading:11760086... | lld:pubmed |
| pubmed-article:11760086 | pubmed:meshHeading | pubmed-meshheading:11760086... | lld:pubmed |
| pubmed-article:11760086 | pubmed:meshHeading | pubmed-meshheading:11760086... | lld:pubmed |
| pubmed-article:11760086 | pubmed:meshHeading | pubmed-meshheading:11760086... | lld:pubmed |
| pubmed-article:11760086 | pubmed:meshHeading | pubmed-meshheading:11760086... | lld:pubmed |
| pubmed-article:11760086 | pubmed:meshHeading | pubmed-meshheading:11760086... | lld:pubmed |
| pubmed-article:11760086 | pubmed:meshHeading | pubmed-meshheading:11760086... | lld:pubmed |
| pubmed-article:11760086 | pubmed:meshHeading | pubmed-meshheading:11760086... | lld:pubmed |
| pubmed-article:11760086 | pubmed:meshHeading | pubmed-meshheading:11760086... | lld:pubmed |
| pubmed-article:11760086 | pubmed:meshHeading | pubmed-meshheading:11760086... | lld:pubmed |
| pubmed-article:11760086 | pubmed:meshHeading | pubmed-meshheading:11760086... | lld:pubmed |
| pubmed-article:11760086 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11760086 | pubmed:articleTitle | Purified canine CD34+Lin- marrow cells transduced with retroviral vectors give rise to long-term multi-lineage hematopoiesis. | lld:pubmed |
| pubmed-article:11760086 | pubmed:affiliation | Clinical Research Division, Fred Hutchinson Cancer Research Center, University of Washington Medical School, Seattle, USA. | lld:pubmed |
| pubmed-article:11760086 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11760086 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:11760086 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:11760086 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
