| pubmed-article:11751621 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11751621 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:11751621 | lifeskim:mentions | umls-concept:C0010531 | lld:lifeskim |
| pubmed-article:11751621 | lifeskim:mentions | umls-concept:C0441889 | lld:lifeskim |
| pubmed-article:11751621 | lifeskim:mentions | umls-concept:C0442805 | lld:lifeskim |
| pubmed-article:11751621 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:11751621 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:11751621 | pubmed:dateCreated | 2001-12-25 | lld:pubmed |
| pubmed-article:11751621 | pubmed:abstractText | The orphan nuclear receptor steroidogenic factor 1 (SF-1) is an essential regulator of endocrine organogenesis, sexual differentiation, and steroidogenesis. SF-1 is a transcriptional regulator of cAMP responsive genes, but the exact mechanisms by which cAMP-dependent PKA modulates SF-1 dependent transcription leading to increased steroidogenic output have not been determined. In this report the effects of PKA activation on SF-1 in living cells have been examined by the use of full-length SF-1 cDNA fused to the cDNA encoding green fluorescent protein (GFP). The GFP-SF-1 fusion protein localized to the nucleus of both steroidogenic Y1 cells and nonsteroidogenic COS-1 cells, and the functional properties of wild-type SF-1 were conserved. When the catalytic subunit of PKA was coexpressed with GFP-SF-1, we observed that the fluorescence emission was markedly elevated. These findings were confirmed by Western blot analysis, showing that stimulation of PKA increased SF-1 protein levels. The PKA- induced expression of SF-1 protein was not accompanied by an increase in SF-1 mRNA levels. However, pulse-chase studies showed a decrease in SF-1 degradation rate in response to activation of PKA, indicating that PKA elevates the level of SF-1 by increasing the stability of SF-1 protein. | lld:pubmed |
| pubmed-article:11751621 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11751621 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11751621 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:11751621 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11751621 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11751621 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11751621 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11751621 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11751621 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11751621 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11751621 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11751621 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11751621 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11751621 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11751621 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11751621 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11751621 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:11751621 | pubmed:issn | 0013-7227 | lld:pubmed |
| pubmed-article:11751621 | pubmed:author | pubmed-author:MellgrenGunna... | lld:pubmed |
| pubmed-article:11751621 | pubmed:author | pubmed-author:AesøyReidunR | lld:pubmed |
| pubmed-article:11751621 | pubmed:author | pubmed-author:MorohashiKen-... | lld:pubmed |
| pubmed-article:11751621 | pubmed:author | pubmed-author:LundJohanJ | lld:pubmed |
| pubmed-article:11751621 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11751621 | pubmed:volume | 143 | lld:pubmed |
| pubmed-article:11751621 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11751621 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11751621 | pubmed:pagination | 295-303 | lld:pubmed |
| pubmed-article:11751621 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
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| pubmed-article:11751621 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:11751621 | pubmed:articleTitle | Activation of cAMP-dependent protein kinase increases the protein level of steroidogenic factor-1. | lld:pubmed |
| pubmed-article:11751621 | pubmed:affiliation | Department of Anatomy and Cell Biology, University of Bergen, Aarstadveien 19, N-5009 Bergen, Norway. reidun.asoy@pki.uib.no | lld:pubmed |
| pubmed-article:11751621 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11751621 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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