| pubmed-article:11744631 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11744631 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:11744631 | lifeskim:mentions | umls-concept:C0521346 | lld:lifeskim |
| pubmed-article:11744631 | lifeskim:mentions | umls-concept:C0761329 | lld:lifeskim |
| pubmed-article:11744631 | lifeskim:mentions | umls-concept:C0026682 | lld:lifeskim |
| pubmed-article:11744631 | lifeskim:mentions | umls-concept:C0017968 | lld:lifeskim |
| pubmed-article:11744631 | lifeskim:mentions | umls-concept:C1417498 | lld:lifeskim |
| pubmed-article:11744631 | lifeskim:mentions | umls-concept:C0004083 | lld:lifeskim |
| pubmed-article:11744631 | lifeskim:mentions | umls-concept:C1521827 | lld:lifeskim |
| pubmed-article:11744631 | lifeskim:mentions | umls-concept:C1314939 | lld:lifeskim |
| pubmed-article:11744631 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:11744631 | lifeskim:mentions | umls-concept:C0332120 | lld:lifeskim |
| pubmed-article:11744631 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:11744631 | pubmed:dateCreated | 2001-12-17 | lld:pubmed |
| pubmed-article:11744631 | pubmed:abstractText | Rate-zonal centrifugation of a reduced and alkylated respiratory mucin preparation identified a protein-rich fraction. This was subjected to trypsin treatment and one of the many liberated peptides was purified and its N-terminal sequence determined. The peptide was identical to a 14 amino acid sequence from the scavenger receptor cysteine-rich domain containing glycoprotein gp-340. A polyclonal antiserum, raised against the peptide, stained the serous cells in the submucosal glands of human tracheal tissue. The glycoprotein was purified from respiratory mucus by density-gradient centrifugation, gel chromatography, and anion exchange chromatography. The molecule exhibited a heterogeneous distribution of buoyant density (1.28-1.46 g/ml) that overlapped with the gel-forming mucins, was included on Sepharose CL-2B and was quite highly anionic. SDS-PAGE indicated a mass greater than 208 kDa and measurements performed across the molecular size distribution indicated an average M(r) of 5 x 10(5) with a range of M(r) from 2 x 10(5) to 1 x 10(6). Gel chromatography of respiratory mucus extracts ("associative" and "dissociative") indicated that this glycoprotein forms complexes that may involve the large gel-forming mucins MUC5AC and MUC5B. Rate zonal centrifugation suggested such complexes are more likely to involve MUC5B rather than MUC5AC mucins. | lld:pubmed |
| pubmed-article:11744631 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11744631 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11744631 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11744631 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11744631 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11744631 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11744631 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11744631 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11744631 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11744631 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11744631 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11744631 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11744631 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:11744631 | pubmed:issn | 0959-6658 | lld:pubmed |
| pubmed-article:11744631 | pubmed:author | pubmed-author:RichardsonP... | lld:pubmed |
| pubmed-article:11744631 | pubmed:author | pubmed-author:SheehanJ KJK | lld:pubmed |
| pubmed-article:11744631 | pubmed:author | pubmed-author:DaviesJ RJR | lld:pubmed |
| pubmed-article:11744631 | pubmed:author | pubmed-author:KhanNN | lld:pubmed |
| pubmed-article:11744631 | pubmed:author | pubmed-author:KirkhamSS | lld:pubmed |
| pubmed-article:11744631 | pubmed:author | pubmed-author:ThorntonD JDJ | lld:pubmed |
| pubmed-article:11744631 | pubmed:author | pubmed-author:GautreyAA | lld:pubmed |
| pubmed-article:11744631 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11744631 | pubmed:volume | 11 | lld:pubmed |
| pubmed-article:11744631 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11744631 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11744631 | pubmed:pagination | 969-77 | lld:pubmed |
| pubmed-article:11744631 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:11744631 | pubmed:meshHeading | pubmed-meshheading:11744631... | lld:pubmed |
| pubmed-article:11744631 | pubmed:meshHeading | pubmed-meshheading:11744631... | lld:pubmed |
| pubmed-article:11744631 | pubmed:meshHeading | pubmed-meshheading:11744631... | lld:pubmed |
| pubmed-article:11744631 | pubmed:meshHeading | pubmed-meshheading:11744631... | lld:pubmed |
| pubmed-article:11744631 | pubmed:meshHeading | pubmed-meshheading:11744631... | lld:pubmed |
| pubmed-article:11744631 | pubmed:meshHeading | pubmed-meshheading:11744631... | lld:pubmed |
| pubmed-article:11744631 | pubmed:meshHeading | pubmed-meshheading:11744631... | lld:pubmed |
| pubmed-article:11744631 | pubmed:meshHeading | pubmed-meshheading:11744631... | lld:pubmed |
| pubmed-article:11744631 | pubmed:meshHeading | pubmed-meshheading:11744631... | lld:pubmed |
| pubmed-article:11744631 | pubmed:meshHeading | pubmed-meshheading:11744631... | lld:pubmed |
| pubmed-article:11744631 | pubmed:meshHeading | pubmed-meshheading:11744631... | lld:pubmed |
| pubmed-article:11744631 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11744631 | pubmed:articleTitle | Identification of a nonmucin glycoprotein (gp-340) from a purified respiratory mucin preparation: evidence for an association involving the MUC5B mucin. | lld:pubmed |
| pubmed-article:11744631 | pubmed:affiliation | Wellcome Trust Centre for Cell-Matrix Research, Division of Biochemistry, School of Biological Sciences, 2.205 Stopford Building, University of Manchester, Manchester, M13 9PT, UK. | lld:pubmed |
| pubmed-article:11744631 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11744631 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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