| pubmed-article:11741930 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11741930 | lifeskim:mentions | umls-concept:C0006069 | lld:lifeskim |
| pubmed-article:11741930 | lifeskim:mentions | umls-concept:C0205147 | lld:lifeskim |
| pubmed-article:11741930 | lifeskim:mentions | umls-concept:C0076925 | lld:lifeskim |
| pubmed-article:11741930 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:11741930 | lifeskim:mentions | umls-concept:C0017376 | lld:lifeskim |
| pubmed-article:11741930 | lifeskim:mentions | umls-concept:C0023978 | lld:lifeskim |
| pubmed-article:11741930 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:11741930 | lifeskim:mentions | umls-concept:C0949655 | lld:lifeskim |
| pubmed-article:11741930 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:11741930 | pubmed:dateCreated | 2002-3-11 | lld:pubmed |
| pubmed-article:11741930 | pubmed:abstractText | The bovine leukemia virus (BLV) promoter is located in its 5'-long terminal repeat and is composed of the U3, R, and U5 regions. BLV transcription is regulated by cis-acting elements located in the U3 region, including three 21-bp enhancers required for transactivation of the BLV promoter by the virus-encoded transactivator Tax(BLV). In addition to the U3 cis-acting elements, both the R and U5 regions contain stimulatory sequences. To date, no transcription factor-binding site has been identified in the R region. Here sequence analysis of this region revealed the presence of a potential E box motif (5'-CACGTG-3'). By competition and supershift gel shift assays, we demonstrated that the basic helix-loop-helix transcription factors USF1 and USF2 specifically interacted with this R region E box motif. Mutations abolishing upstream stimulatory factor (USF) binding caused a reproducible decrease in basal or Tax-activated BLV promoter-driven gene expression in transient transfection assays of B-lymphoid cell lines. Cotransfection experiments showed that the USF1 and USF2a transactivators were able to act through the BLV R region E box. Taken together, these results physically and functionally characterize a USF-binding site in the R region of BLV. This E box motif located downstream of the transcription start site constitutes a new positive regulatory element involved in the transcriptional activity of the BLV promoter and could play an important role in virus replication. | lld:pubmed |
| pubmed-article:11741930 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11741930 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11741930 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11741930 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11741930 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11741930 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11741930 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11741930 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11741930 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11741930 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:11741930 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:11741930 | pubmed:author | pubmed-author:de... | lld:pubmed |
| pubmed-article:11741930 | pubmed:author | pubmed-author:CalommeClaire... | lld:pubmed |
| pubmed-article:11741930 | pubmed:author | pubmed-author:NguyenThi... | lld:pubmed |
| pubmed-article:11741930 | pubmed:author | pubmed-author:KiermerVeroni... | lld:pubmed |
| pubmed-article:11741930 | pubmed:author | pubmed-author:DroogmansLoui... | lld:pubmed |
| pubmed-article:11741930 | pubmed:author | pubmed-author:BurnyArseneA | lld:pubmed |
| pubmed-article:11741930 | pubmed:author | pubmed-author:Van... | lld:pubmed |
| pubmed-article:11741930 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11741930 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:11741930 | pubmed:volume | 277 | lld:pubmed |
| pubmed-article:11741930 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11741930 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11741930 | pubmed:pagination | 8775-89 | lld:pubmed |
| pubmed-article:11741930 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:11741930 | pubmed:meshHeading | pubmed-meshheading:11741930... | lld:pubmed |
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| pubmed-article:11741930 | pubmed:meshHeading | pubmed-meshheading:11741930... | lld:pubmed |
| pubmed-article:11741930 | pubmed:meshHeading | pubmed-meshheading:11741930... | lld:pubmed |
| pubmed-article:11741930 | pubmed:meshHeading | pubmed-meshheading:11741930... | lld:pubmed |
| pubmed-article:11741930 | pubmed:meshHeading | pubmed-meshheading:11741930... | lld:pubmed |
| pubmed-article:11741930 | pubmed:meshHeading | pubmed-meshheading:11741930... | lld:pubmed |
| pubmed-article:11741930 | pubmed:meshHeading | pubmed-meshheading:11741930... | lld:pubmed |
| pubmed-article:11741930 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:11741930 | pubmed:articleTitle | Upstream stimulatory factors binding to an E box motif in the R region of the bovine leukemia virus long terminal repeat stimulates viral gene expression. | lld:pubmed |
| pubmed-article:11741930 | pubmed:affiliation | Université Libre de Bruxelles, Institut de Biologie et de Médecine Moléculaires, Service de Chimie Biologique, Laboratoire de Virologie Moléculaire, Rue des Profs Jeener et Brachet 12, 6041 Gosselies, Belgium. | lld:pubmed |
| pubmed-article:11741930 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11741930 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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