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pubmed-article:11724876pubmed:abstractTextMethicillin-resistant Staphylococcus aureus septicemia is associated with significant morbidity and mortality and requires treatment with intravenous glycopeptides. For blood cultures positive for gram-positive cocci, 24 to 48 h is required for the detection of S. aureus bacteremia and the provision of antibiotic susceptibility testing results. We describe a molecular biology-based assay that requires 2 h from the time of initial positivity of blood cultures. The assay correctly detected 96% of the S. aureus isolates including all methicillin-resistant S. aureus isolates. Clinical data collected during the study suggest that 28% of patients with S. aureus bacteremia do not receive early and appropriate treatment and that 10% of patients may initially be receiving inappropriate glycopeptide treatment.lld:pubmed
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pubmed-article:11724876pubmed:articleTitleRapid identification of methicillin-resistant Staphylococcus aureus from positive blood cultures by real-time fluorescence PCR.lld:pubmed
pubmed-article:11724876pubmed:affiliationDepartment of Microbiology, Public Health Laboratory Service, University Hospital Wales, Cardiff, United Kingdom. theanyen.tan@phls.wales.nhs.uklld:pubmed
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