| pubmed-article:11698234 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11698234 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:11698234 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:11698234 | lifeskim:mentions | umls-concept:C1267092 | lld:lifeskim |
| pubmed-article:11698234 | lifeskim:mentions | umls-concept:C0007770 | lld:lifeskim |
| pubmed-article:11698234 | lifeskim:mentions | umls-concept:C1515877 | lld:lifeskim |
| pubmed-article:11698234 | lifeskim:mentions | umls-concept:C0439799 | lld:lifeskim |
| pubmed-article:11698234 | lifeskim:mentions | umls-concept:C0439300 | lld:lifeskim |
| pubmed-article:11698234 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:11698234 | lifeskim:mentions | umls-concept:C0332324 | lld:lifeskim |
| pubmed-article:11698234 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:11698234 | pubmed:dateCreated | 2001-11-7 | lld:pubmed |
| pubmed-article:11698234 | pubmed:abstractText | The goal of the present study was to test the hypothesis that local Ca(2+) release events (Ca(2+) sparks) deliver high local Ca(2+) concentration to activate nearby Ca(2+)-sensitive K(+) (BK) channels in the cell membrane of arterial smooth muscle cells. Ca(2+) sparks and BK channels were examined in isolated myocytes from rat cerebral arteries with laser scanning confocal microscopy and patch-clamp techniques. BK channels had an apparent dissociation constant for Ca(2+) of 19 microM and a Hill coefficient of 2.9 at -40 mV. At near-physiological intracellular Ca(2+) concentration ([Ca(2+)](i); 100 nM) and membrane potential (-40 mV), the open probability of a single BK channel was low (1.2 x 10(-6)). A Ca(2+) spark increased BK channel activity to 18. Assuming that 1-100% of the BK channels are activated by a single Ca(2+) spark, BK channel activity increases 6 x 10(5)-fold to 6 x 10(3)-fold, which corresponds to approximately 30 microM to 4 microM spark Ca(2+) concentration. 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid acetoxymethyl ester caused the disappearance of all Ca(2+) sparks while leaving the transient BK currents unchanged. Our results support the idea that Ca(2+) spark sites are in close proximity to the BK channels and that local [Ca(2+)](i) reaches micromolar levels to activate BK channels. | lld:pubmed |
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| pubmed-article:11698234 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11698234 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11698234 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11698234 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:11698234 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11698234 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:11698234 | pubmed:issn | 0363-6143 | lld:pubmed |
| pubmed-article:11698234 | pubmed:author | pubmed-author:NelsonM TMT | lld:pubmed |
| pubmed-article:11698234 | pubmed:author | pubmed-author:BonevA DAD | lld:pubmed |
| pubmed-article:11698234 | pubmed:author | pubmed-author:PérezG JGJ | lld:pubmed |
| pubmed-article:11698234 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11698234 | pubmed:volume | 281 | lld:pubmed |
| pubmed-article:11698234 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11698234 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11698234 | pubmed:pagination | C1769-75 | lld:pubmed |
| pubmed-article:11698234 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:11698234 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11698234 | pubmed:articleTitle | Micromolar Ca(2+) from sparks activates Ca(2+)-sensitive K(+) channels in rat cerebral artery smooth muscle. | lld:pubmed |
| pubmed-article:11698234 | pubmed:affiliation | Department of Pharmacology, University of Vermont, Burlington, Vermont 05405, USA. | lld:pubmed |
| pubmed-article:11698234 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11698234 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:11698234 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:11698234 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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