| pubmed-article:11698172 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11698172 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:11698172 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:11698172 | lifeskim:mentions | umls-concept:C0022567 | lld:lifeskim |
| pubmed-article:11698172 | lifeskim:mentions | umls-concept:C0043335 | lld:lifeskim |
| pubmed-article:11698172 | lifeskim:mentions | umls-concept:C0205390 | lld:lifeskim |
| pubmed-article:11698172 | lifeskim:mentions | umls-concept:C0243102 | lld:lifeskim |
| pubmed-article:11698172 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:11698172 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:11698172 | pubmed:dateCreated | 2001-11-7 | lld:pubmed |
| pubmed-article:11698172 | pubmed:abstractText | This study analyses the expression and induction of several drug-metabolising enzyme activities involved in either phase I or phase II biotransformations in NCTC 2544 human keratinocytes. The phase I activities 7-ethoxycoumarin O-deethylase (ECOD), 7-ethoxyresorufin O-deethylase (EROD) and 7-pentoxyresorufin O-depenthylase (PROD) were easily detectable in basal conditions. During incubations lasting up to 144 h in the presence of the classical cytochrome P450 inducers beta-naphthoflavone (BNF), 3-methylcholanthrene (MC) and phenobarbital (PB), a considerable and significant increase in all the three activities was observed. PROD activity was induced up to 4.5-fold after 96 h in the presence of PB. The MC-induced ECOD and EROD activities were also dose-dependently inhibited by alpha-naphothflavone, which was given to the cells during the incubation with CYP 1A1 inducers. Also the PB-induced PROD activity was decreased by the simultaneous addition of the CYP 2B inhibitor metyrapone. Both cytochrome P450 inhibitors were used at non-cytotoxic concentrations. The phase II enzymes glutathione S-transferase, aldehyde dehydrogenase and quinone reductase were all highly expressed and inducible by MC. The exposure (24 h) of the cells to four hair dyes used in cosmetic formulations resulted in a marked increase in ECOD activity. All data give sustained evidence for the suitability of NCTC 2544 cell line to skin toxicology studies. | lld:pubmed |
| pubmed-article:11698172 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11698172 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11698172 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11698172 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11698172 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11698172 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11698172 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11698172 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11698172 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11698172 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11698172 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11698172 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11698172 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:11698172 | pubmed:issn | 0887-2333 | lld:pubmed |
| pubmed-article:11698172 | pubmed:author | pubmed-author:MorinoHH | lld:pubmed |
| pubmed-article:11698172 | pubmed:author | pubmed-author:PencoSS | lld:pubmed |
| pubmed-article:11698172 | pubmed:author | pubmed-author:FerroMM | lld:pubmed |
| pubmed-article:11698172 | pubmed:author | pubmed-author:GelardiAA | lld:pubmed |
| pubmed-article:11698172 | pubmed:author | pubmed-author:DusattiFF | lld:pubmed |
| pubmed-article:11698172 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11698172 | pubmed:volume | 15 | lld:pubmed |
| pubmed-article:11698172 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11698172 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11698172 | pubmed:pagination | 701-11 | lld:pubmed |
| pubmed-article:11698172 | pubmed:dateRevised | 2009-4-10 | lld:pubmed |
| pubmed-article:11698172 | pubmed:meshHeading | pubmed-meshheading:11698172... | lld:pubmed |
| pubmed-article:11698172 | pubmed:meshHeading | pubmed-meshheading:11698172... | lld:pubmed |
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| pubmed-article:11698172 | pubmed:meshHeading | pubmed-meshheading:11698172... | lld:pubmed |
| pubmed-article:11698172 | pubmed:meshHeading | pubmed-meshheading:11698172... | lld:pubmed |
| pubmed-article:11698172 | pubmed:meshHeading | pubmed-meshheading:11698172... | lld:pubmed |
| pubmed-article:11698172 | pubmed:meshHeading | pubmed-meshheading:11698172... | lld:pubmed |
| pubmed-article:11698172 | pubmed:meshHeading | pubmed-meshheading:11698172... | lld:pubmed |
| pubmed-article:11698172 | pubmed:meshHeading | pubmed-meshheading:11698172... | lld:pubmed |
| pubmed-article:11698172 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11698172 | pubmed:articleTitle | Induction by xenobiotics of phase I and phase II enzyme activities in the human keratinocyte cell line NCTC 2544. | lld:pubmed |
| pubmed-article:11698172 | pubmed:affiliation | Department of Experimental Medicine, General Pathology Division, University of Genoa, Via L.B. Alberti, 2, 16132, Genova, Italy. | lld:pubmed |
| pubmed-article:11698172 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11698172 | lld:pubmed |
