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pubmed-article:11693295pubmed:dateCreated2001-11-5lld:pubmed
pubmed-article:11693295pubmed:abstractTextA study was conducted to determine the location and distribution of PAH and PAH-degrading bacteria in different aggregate size fractions of an industrially polluted soil. The estimation of PAH-degrading bacteria using an MPN microplate technique indicated that these bacteria are most numerous in the aggregate size fractions corresponding to fine silt (2-20 microm) and clay (<2 microm) compared to larger fractions or unfractionated soil. PAH concentrations were also highest in the aggregate size fraction corresponding to fine silt. Similar results were found in a spiked soil (incubated for 6 months) with similar carbonated minerals. Transmission electron microscopy observations showed that the autochtonous PAH-degrading bacteria were embedded in the aggregates where PAHs were abundant. In spite of this extensive co-localisation PAH degradation was limited during 6 months incubation. This indicates that factors other than spatial distribution and PAH degrading ability control degradation rates. The fine silt fraction of the industrial soil had an elevated C/N ratio (35) compared to the clay fraction (C/N: 16). Thus the fraction which assumably had the highest specific surface area contained less PAH but similar numbers of PAH-degraders. N thus seem to play an important role in the long term, but as PAH degradation was low in fine size fractions, other sources/factors were probably limiting (easily degradable C, P org, O2 etc.). Based on these findings, soil particle organization and structure of soil aggregates appear to be important for the characterization of a polluted soil (localization and sequestration). Manipulations that modify aggregation in polluted soils could thus potentially influence the accessibility and biodegradability of PAHs.lld:pubmed
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pubmed-article:11693295pubmed:authorpubmed-author:VogelTTlld:pubmed
pubmed-article:11693295pubmed:authorpubmed-author:AmellalNNlld:pubmed
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pubmed-article:11693295pubmed:authorpubmed-author:BerthelinJJlld:pubmed
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pubmed-article:11693295pubmed:pagination49-57lld:pubmed
pubmed-article:11693295pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:11693295pubmed:year2001lld:pubmed
pubmed-article:11693295pubmed:articleTitleDistribution and location of polycyclic aromatic hydrocarbons (PAHs) and PAH-degrading bacteria within polluted soil aggregates.lld:pubmed
pubmed-article:11693295pubmed:affiliationCentre de Pédologie-Biologique, UPR 6831 du CNRS associée à l'Université' Henri Poincaré-Nancy I, Vandaeuvre-lès-Nancy, France. amellal@cpb.cnrs-nancy.frlld:pubmed
pubmed-article:11693295pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:11693295pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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