| pubmed-article:11691823 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11691823 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:11691823 | lifeskim:mentions | umls-concept:C0812201 | lld:lifeskim |
| pubmed-article:11691823 | lifeskim:mentions | umls-concept:C0017638 | lld:lifeskim |
| pubmed-article:11691823 | lifeskim:mentions | umls-concept:C0007577 | lld:lifeskim |
| pubmed-article:11691823 | lifeskim:mentions | umls-concept:C0298340 | lld:lifeskim |
| pubmed-article:11691823 | lifeskim:mentions | umls-concept:C0013081 | lld:lifeskim |
| pubmed-article:11691823 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:11691823 | lifeskim:mentions | umls-concept:C0376315 | lld:lifeskim |
| pubmed-article:11691823 | lifeskim:mentions | umls-concept:C0600210 | lld:lifeskim |
| pubmed-article:11691823 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:11691823 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:11691823 | pubmed:issue | 21 | lld:pubmed |
| pubmed-article:11691823 | pubmed:dateCreated | 2001-11-5 | lld:pubmed |
| pubmed-article:11691823 | pubmed:abstractText | Ets transcription factors are associated with tumor malignancy. We reported previously that the stable transfection of the dominant-negative form of Ets-1 (Ets-DN) in the glioma cell line U251 induced down-regulation of urokinase-type plasminogen activator mRNA expression and invasiveness (M. Nakada et al., J. Neuropathol. Exp. Neurol., 58: 329-334, 1999). Here we analyzed effects of Ets-DN expression on cell adhesion, migration, and phosphorylation of focal adhesion kinase. U251 cells expressing Ets-DN (U251-DN) showed reduced cell adhesion, spreading, and extension of actin stress fibers on dishes coated with fibronectin but not on dishes coated with collagen. Migration of U251-DN cells was found to be significantly inhibited compared with that of parental cells when examined by wound-induced migration assay on fibronectin-coated dishes. Phosphorylation levels of focal adhesion kinase in U251-DN cells were also attenuated on dishes coated with fibronectin. Reduced expression level of integrin alpha5 subunit in U251-DN cells was demonstrated by semiquantitative reverse transcription-PCR analysis. Semiquantitative reverse transcription-PCR of surgical samples of brain tumors revealed that the expression level of Ets-1 mRNA correlated with that of integrin alpha5 mRNA in glioma. The experimental metastatic ability of U251-DN cells examined in chick embryo was considerably lower than that of parental cells. These results suggest that Ets-1 contributes to glioma malignancy by up- regulating expression of the integrin alpha5 subunit, which composes integrin alpha5beta1 and mediates intracellular signaling and the subsequent acceleration of the invasive process, including cell adhesion and migration. | lld:pubmed |
| pubmed-article:11691823 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11691823 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11691823 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11691823 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11691823 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11691823 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11691823 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11691823 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:11691823 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:11691823 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11691823 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11691823 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11691823 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11691823 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11691823 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11691823 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:11691823 | pubmed:issn | 0008-5472 | lld:pubmed |
| pubmed-article:11691823 | pubmed:author | pubmed-author:TakahashiTT | lld:pubmed |
| pubmed-article:11691823 | pubmed:author | pubmed-author:YamashitaJJ | lld:pubmed |
| pubmed-article:11691823 | pubmed:author | pubmed-author:SatoHH | lld:pubmed |
| pubmed-article:11691823 | pubmed:author | pubmed-author:TakinoTT | lld:pubmed |
| pubmed-article:11691823 | pubmed:author | pubmed-author:NakadaMM | lld:pubmed |
| pubmed-article:11691823 | pubmed:author | pubmed-author:KitaDD | lld:pubmed |
| pubmed-article:11691823 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11691823 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:11691823 | pubmed:volume | 61 | lld:pubmed |
| pubmed-article:11691823 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11691823 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11691823 | pubmed:pagination | 7985-91 | lld:pubmed |
| pubmed-article:11691823 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:11691823 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11691823 | pubmed:articleTitle | Expression of dominant-negative form of Ets-1 suppresses fibronectin-stimulated cell adhesion and migration through down-regulation of integrin alpha5 expression in U251 glioma cell line. | lld:pubmed |
| pubmed-article:11691823 | pubmed:affiliation | Department of Molecular Virology and Oncology, Kanazawa University, Kanazawa, Ishikawa 920-0934, Japan. | lld:pubmed |
| pubmed-article:11691823 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11691823 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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