| pubmed-article:11677235 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11677235 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:11677235 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:11677235 | lifeskim:mentions | umls-concept:C0015296 | lld:lifeskim |
| pubmed-article:11677235 | lifeskim:mentions | umls-concept:C0441472 | lld:lifeskim |
| pubmed-article:11677235 | lifeskim:mentions | umls-concept:C0675096 | lld:lifeskim |
| pubmed-article:11677235 | lifeskim:mentions | umls-concept:C0683598 | lld:lifeskim |
| pubmed-article:11677235 | lifeskim:mentions | umls-concept:C1158535 | lld:lifeskim |
| pubmed-article:11677235 | lifeskim:mentions | umls-concept:C1709915 | lld:lifeskim |
| pubmed-article:11677235 | lifeskim:mentions | umls-concept:C1883709 | lld:lifeskim |
| pubmed-article:11677235 | lifeskim:mentions | umls-concept:C0178645 | lld:lifeskim |
| pubmed-article:11677235 | pubmed:issue | 52 | lld:pubmed |
| pubmed-article:11677235 | pubmed:dateCreated | 2001-12-25 | lld:pubmed |
| pubmed-article:11677235 | pubmed:abstractText | Cyclopurine deoxynucleosides are common DNA lesions generated by exposure to reactive oxygen species under hypoxic conditions. The S and R diastereoisomers of cyclodeoxyadenosine on DNA were investigated separately for their ability to block 3' to 5' exonucleases. The mammalian DNA-editing enzyme DNase III (TREX1) was blocked by both diastereoisomers, whereas only the S diastereoisomer was highly efficient in preventing digestion by the exonuclease function of T4 DNA polymerase. Digestion in both cases was frequently blocked one residue before the modified base. Oligodeoxyribonucleotides containing a cyclodeoxyadenosine residue were further employed as templates for synthesis by human DNA polymerase eta (pol eta). pol eta could catalyze translesion synthesis on the R diastereoisomer of cyclodeoxyadenosine. On the S diastereoisomer, pol eta could catalyze the incorporation of one nucleotide opposite the lesion but could not continue elongation. Although pol eta preferentially incorporated dAMP opposite the R diastereoisomer, elongation continued only when dTMP was incorporated, suggesting bypass of this lesion by pol eta with reasonable fidelity. With the S diastereoisomer, pol eta mainly incorporated dAMP or dTMP opposite the lesion but could not elongate even after incorporating a correct nucleotide. These data suggest that the S diastereoisomer may be a more cytotoxic DNA lesion than the R diastereoisomer. | lld:pubmed |
| pubmed-article:11677235 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11677235 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11677235 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11677235 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11677235 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:11677235 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11677235 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11677235 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:11677235 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:11677235 | pubmed:author | pubmed-author:LindahlTT | lld:pubmed |
| pubmed-article:11677235 | pubmed:author | pubmed-author:RobinsPP | lld:pubmed |
| pubmed-article:11677235 | pubmed:author | pubmed-author:CadetJJ | lld:pubmed |
| pubmed-article:11677235 | pubmed:author | pubmed-author:HanaokaFF | lld:pubmed |
| pubmed-article:11677235 | pubmed:author | pubmed-author:WoodR DRD | lld:pubmed |
| pubmed-article:11677235 | pubmed:author | pubmed-author:GasparuttoDD | lld:pubmed |
| pubmed-article:11677235 | pubmed:author | pubmed-author:MasutaniCC | lld:pubmed |
| pubmed-article:11677235 | pubmed:author | pubmed-author:KuraokaII | lld:pubmed |
| pubmed-article:11677235 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11677235 | pubmed:day | 28 | lld:pubmed |
| pubmed-article:11677235 | pubmed:volume | 276 | lld:pubmed |
| pubmed-article:11677235 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11677235 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11677235 | pubmed:pagination | 49283-8 | lld:pubmed |
| pubmed-article:11677235 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:11677235 | pubmed:meshHeading | pubmed-meshheading:11677235... | lld:pubmed |
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| pubmed-article:11677235 | pubmed:meshHeading | pubmed-meshheading:11677235... | lld:pubmed |
| pubmed-article:11677235 | pubmed:meshHeading | pubmed-meshheading:11677235... | lld:pubmed |
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| pubmed-article:11677235 | pubmed:meshHeading | pubmed-meshheading:11677235... | lld:pubmed |
| pubmed-article:11677235 | pubmed:meshHeading | pubmed-meshheading:11677235... | lld:pubmed |
| pubmed-article:11677235 | pubmed:meshHeading | pubmed-meshheading:11677235... | lld:pubmed |
| pubmed-article:11677235 | pubmed:meshHeading | pubmed-meshheading:11677235... | lld:pubmed |
| pubmed-article:11677235 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11677235 | pubmed:articleTitle | Oxygen free radical damage to DNA. Translesion synthesis by human DNA polymerase eta and resistance to exonuclease action at cyclopurine deoxynucleoside residues. | lld:pubmed |
| pubmed-article:11677235 | pubmed:affiliation | Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, United Kingdom. | lld:pubmed |
| pubmed-article:11677235 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11677235 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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