| pubmed-article:11595752 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11595752 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:11595752 | lifeskim:mentions | umls-concept:C0032043 | lld:lifeskim |
| pubmed-article:11595752 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:11595752 | lifeskim:mentions | umls-concept:C0008407 | lld:lifeskim |
| pubmed-article:11595752 | lifeskim:mentions | umls-concept:C0015295 | lld:lifeskim |
| pubmed-article:11595752 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:11595752 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:11595752 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:11595752 | pubmed:dateCreated | 2001-10-11 | lld:pubmed |
| pubmed-article:11595752 | pubmed:abstractText | To investigate the existence of 5'-region(s) of human choline acetyltransferase (hChAT) mRNA in placenta we analyzed the presence or absence of ChAT 5'-untranslated regions (UTR) in human neuronal and non-neuronal cells. Total RNA from human spinal cord, placenta, cultured choriocarcinoma JEG-3 and neuroblastoma CHP126 and MC-IXC cells was reverse transcribed and used for polymerase chain reaction amplification (RT-PCR). We used a sense primer located in the 5'-flanking region, in the previously defined intronic sequence and an anti-sense primer located in the common coding exon 2 of the hChAT gene. An amplified product of 567 bp in size was obtained only in human placenta and in JEG-3 cells whereas it was absent in spinal cord, CHP126 and MC-IXC cells. It was designated 'H-type' of ChAT mRNA. Whereas CHP126 produced the R- and N-type of ChAT mRNAs, no transcript of the N-and R-type was detected in JEG-3 and human placenta. In addition, CHP126 and JEG-3 cells and placenta showed the expression of the M-type of ChAT mRNA. The identity of the amplified 567 bp product (H-type) was confirmed by Southern hybridization and sequencing. The nucleotide sequence of the amplified fragment in placenta revealed the existence of a previously unknown type of ChAT mRNA produced by alternative splicing. Using primer extension we further determined the transcription initiation site of the H-type hChAT mRNA in placenta. These results demonstrate the expression of a novel ChAT mRNA isoform in human placenta in addition to the M-type. These data may be possibly explained by the presence of a placenta specific promoter in the ChAT gene, which might be the proximal promoter P1. | lld:pubmed |
| pubmed-article:11595752 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11595752 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11595752 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11595752 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11595752 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11595752 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11595752 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:11595752 | pubmed:issn | 0022-3042 | lld:pubmed |
| pubmed-article:11595752 | pubmed:author | pubmed-author:Quirin-Strick... | lld:pubmed |
| pubmed-article:11595752 | pubmed:author | pubmed-author:RobertII | lld:pubmed |
| pubmed-article:11595752 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11595752 | pubmed:volume | 79 | lld:pubmed |
| pubmed-article:11595752 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11595752 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11595752 | pubmed:pagination | 9-16 | lld:pubmed |
| pubmed-article:11595752 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:11595752 | pubmed:meshHeading | pubmed-meshheading:11595752... | lld:pubmed |
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| pubmed-article:11595752 | pubmed:meshHeading | pubmed-meshheading:11595752... | lld:pubmed |
| pubmed-article:11595752 | pubmed:meshHeading | pubmed-meshheading:11595752... | lld:pubmed |
| pubmed-article:11595752 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11595752 | pubmed:articleTitle | A novel untranslated 'exon H' of the human choline acetyltransferase gene in placenta. | lld:pubmed |
| pubmed-article:11595752 | pubmed:affiliation | Laboratoire de Génétique Moléculaire des Eucaryotes, CNRS, Biologie Moléculaire et de Génie Génétique, INSERM, Institut de Chimie Biologique, Faculté de Médecine, Strasbourg, France. | lld:pubmed |
| pubmed-article:11595752 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11595752 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:1103 | entrezgene:pubmed | pubmed-article:11595752 | lld:entrezgene |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11595752 | lld:pubmed |
