| pubmed-article:11581161 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11581161 | lifeskim:mentions | umls-concept:C0035696 | lld:lifeskim |
| pubmed-article:11581161 | lifeskim:mentions | umls-concept:C0142661 | lld:lifeskim |
| pubmed-article:11581161 | lifeskim:mentions | umls-concept:C0029073 | lld:lifeskim |
| pubmed-article:11581161 | lifeskim:mentions | umls-concept:C0439855 | lld:lifeskim |
| pubmed-article:11581161 | lifeskim:mentions | umls-concept:C0600448 | lld:lifeskim |
| pubmed-article:11581161 | lifeskim:mentions | umls-concept:C0162610 | lld:lifeskim |
| pubmed-article:11581161 | lifeskim:mentions | umls-concept:C0332256 | lld:lifeskim |
| pubmed-article:11581161 | lifeskim:mentions | umls-concept:C1522492 | lld:lifeskim |
| pubmed-article:11581161 | lifeskim:mentions | umls-concept:C0444930 | lld:lifeskim |
| pubmed-article:11581161 | pubmed:issue | 19 | lld:pubmed |
| pubmed-article:11581161 | pubmed:dateCreated | 2001-10-2 | lld:pubmed |
| pubmed-article:11581161 | pubmed:abstractText | Polycistronic pre-mRNAs from Caenorhabditis elegans are processed by 3' end formation of the upstream mRNA and SL2-specific trans-splicing of the downstream mRNA. These processes usually occur within an approximately 100-nucleotide region and are mechanistically coupled. In this paper, we report a complex in C. elegans extracts containing the 3' end formation protein CstF-64 and the SL2 snRNP. This complex, immunoprecipitated with alphaCstF-64 antibody, contains SL2 RNA, but not SL1 RNA or other U snRNAs. Using mutational analysis we have been able to uncouple SL2 snRNP function and identity. SL2 RNA with a mutation in stem/loop III is functional in vivo as a trans-splice donor, but fails to splice to SL2-accepting trans-splice sites, suggesting that it has lost its identity as an SL2 snRNP. Importantly, stem/loop III mutations prevent association of SL2 RNA with CstF-64. In contrast, a mutation in stem II that inactivates the SL2 snRNP still permits complex formation with CstF-64. Therefore, SL2 RNA stem/loop III is required for both SL2 identity and formation of a complex containing CstF-64, but not for trans-splicing. These results provide a molecular framework for the coupling of 3' end formation and trans-splicing in the processing of polycistronic pre-mRNAs from C. elegans operons. | lld:pubmed |
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| pubmed-article:11581161 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11581161 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11581161 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11581161 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:11581161 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11581161 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:11581161 | pubmed:issn | 0890-9369 | lld:pubmed |
| pubmed-article:11581161 | pubmed:author | pubmed-author:EvansDD | lld:pubmed |
| pubmed-article:11581161 | pubmed:author | pubmed-author:LeakeDD | lld:pubmed |
| pubmed-article:11581161 | pubmed:author | pubmed-author:BlumenthalTT | lld:pubmed |
| pubmed-article:11581161 | pubmed:author | pubmed-author:PerezII | lld:pubmed |
| pubmed-article:11581161 | pubmed:author | pubmed-author:MacMorrisMM | lld:pubmed |
| pubmed-article:11581161 | pubmed:author | pubmed-author:WiluszC JCJ | lld:pubmed |
| pubmed-article:11581161 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11581161 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:11581161 | pubmed:volume | 15 | lld:pubmed |
| pubmed-article:11581161 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11581161 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11581161 | pubmed:pagination | 2562-71 | lld:pubmed |
| pubmed-article:11581161 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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