| pubmed-article:11534853 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11534853 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:11534853 | lifeskim:mentions | umls-concept:C0010222 | lld:lifeskim |
| pubmed-article:11534853 | lifeskim:mentions | umls-concept:C0597357 | lld:lifeskim |
| pubmed-article:11534853 | lifeskim:mentions | umls-concept:C1171362 | lld:lifeskim |
| pubmed-article:11534853 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:11534853 | lifeskim:mentions | umls-concept:C1515670 | lld:lifeskim |
| pubmed-article:11534853 | lifeskim:mentions | umls-concept:C0205227 | lld:lifeskim |
| pubmed-article:11534853 | lifeskim:mentions | umls-concept:C0675717 | lld:lifeskim |
| pubmed-article:11534853 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:11534853 | pubmed:dateCreated | 2001-9-5 | lld:pubmed |
| pubmed-article:11534853 | pubmed:abstractText | The mammalian G proteins G15 and G16 couple a wide variety of receptors to phospholipase C (PLC) in co-transfected systems, and it has been suggested that they can be used as tools in agonist-screening systems. Using the reversed tetracycline-controlled transactivation system we generated rat pituitary GH3 cell clones that expressed Galphal5 and Galpha16 conditionally to study the coupling of endogenous receptors to both G proteins. In cells expressing moderate levels of Galpha15, activation of various endogenous receptors increased inositol phosphate production, whereas conditional expression of Galpha16 had no significant effect on agonist-dependent PLC activity. Activation of PLC through Galpha15 in response to carbachol did not increase cytosolic [Ca2+] ([Ca2+]i) but stimulated protein kinase C. While carbachol decreased the secretory activity in non-induced GH3 cells, it increased secretion in cells expressing Galpha15. Our data demonstrate that Galpha15 has a higher functional promiscuity than Galpha16 when studied in a system that preserves physiological G protein and receptor levels. In addition, Galpha15-mediated coupling of a receptor to PLC can change the cellular response to receptor agonists, indicating that downstream cellular functions can be used to detect receptor activation in screening systems employing a promiscuous G protein. | lld:pubmed |
| pubmed-article:11534853 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11534853 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11534853 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11534853 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11534853 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11534853 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11534853 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11534853 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11534853 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11534853 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11534853 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11534853 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11534853 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11534853 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11534853 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11534853 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:11534853 | pubmed:issn | 0028-1298 | lld:pubmed |
| pubmed-article:11534853 | pubmed:author | pubmed-author:SimonM IMI | lld:pubmed |
| pubmed-article:11534853 | pubmed:author | pubmed-author:HuY HYH | lld:pubmed |
| pubmed-article:11534853 | pubmed:author | pubmed-author:OffermannsSS | lld:pubmed |
| pubmed-article:11534853 | pubmed:author | pubmed-author:NegulescuPP | lld:pubmed |
| pubmed-article:11534853 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11534853 | pubmed:volume | 364 | lld:pubmed |
| pubmed-article:11534853 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11534853 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11534853 | pubmed:pagination | 140-8 | lld:pubmed |
| pubmed-article:11534853 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:11534853 | pubmed:meshHeading | pubmed-meshheading:11534853... | lld:pubmed |
| pubmed-article:11534853 | pubmed:meshHeading | pubmed-meshheading:11534853... | lld:pubmed |
| pubmed-article:11534853 | pubmed:meshHeading | pubmed-meshheading:11534853... | lld:pubmed |
| pubmed-article:11534853 | pubmed:meshHeading | pubmed-meshheading:11534853... | lld:pubmed |
| pubmed-article:11534853 | pubmed:meshHeading | pubmed-meshheading:11534853... | lld:pubmed |
| pubmed-article:11534853 | pubmed:meshHeading | pubmed-meshheading:11534853... | lld:pubmed |
| pubmed-article:11534853 | pubmed:meshHeading | pubmed-meshheading:11534853... | lld:pubmed |
| pubmed-article:11534853 | pubmed:meshHeading | pubmed-meshheading:11534853... | lld:pubmed |
| pubmed-article:11534853 | pubmed:meshHeading | pubmed-meshheading:11534853... | lld:pubmed |
| pubmed-article:11534853 | pubmed:meshHeading | pubmed-meshheading:11534853... | lld:pubmed |
| pubmed-article:11534853 | pubmed:meshHeading | pubmed-meshheading:11534853... | lld:pubmed |
| pubmed-article:11534853 | pubmed:meshHeading | pubmed-meshheading:11534853... | lld:pubmed |
| pubmed-article:11534853 | pubmed:meshHeading | pubmed-meshheading:11534853... | lld:pubmed |
| pubmed-article:11534853 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11534853 | pubmed:articleTitle | Conditionally expressed G alpha 15 couples to endogenous receptors in GH3 cells. | lld:pubmed |
| pubmed-article:11534853 | pubmed:affiliation | Pharmakologisches Institut, Universität Heidelberg, Germany. Stefan.Offermanns@urz.uni-heidelberg.de | lld:pubmed |
| pubmed-article:11534853 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11534853 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:11534853 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11534853 | lld:pubmed |
