Linked Life Data

11534179

Source:http://linkedlifedata.com/resource/pubmed/id/11534179

Statements in which the resource exists.
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pubmed-article:11534179pubmed:issue6lld:pubmed
pubmed-article:11534179pubmed:dateCreated2001-9-5lld:pubmed
pubmed-article:11534179pubmed:abstractTextA comparative study was made of reactive oxygen species (ROS) in rat embryo fibroblasts and their transformants. Primary rat embryo fibroblasts (REF), REF transformed by the complementing oncogenes E1A plus cHa-ras (cell line E1A + Ras), and REF transformed by E1A plus E1B-19 kDa (cell line E1A + E1B) were studied. ROS generation was measured with microfluorometric assay using fluorescent probe 2',7'-dichlorofluorescin diacetate. It has been shown that the block of REF and E1A + 1B cells in the G1/S under serum-starved conditions (0.5% serum) for 24-48 h was paralleled by a decrease in ROS generation. Activation of serum-starved REF and E1A + 1B cells with 10% serum resulted in reactivation of cell cycle and gradual increase in ROS generation. The maximum intracellular level of ROS correlated in time with the phase of DNA synthesis. Serum-starved E1A + Ras cells were not stopped in the G1/S and ROS production of these cells was not dependent on serum growth factors. The prolonged cultivation of E1A + Ras cells in the medium with low serum content (0.5%) caused a sharp increase in ROS generation, which was accompanied by apoptotic death.lld:pubmed
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pubmed-article:11534179pubmed:volume43lld:pubmed
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pubmed-article:11534179pubmed:pagination595-601lld:pubmed
pubmed-article:11534179pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:11534179pubmed:articleTitle[Specific regulated endonuclease activity of small RNP, containing prosomes from the A431 cell line: possible mechanisms of regulating the stability of RNA by epidermal growth factor].lld:pubmed
pubmed-article:11534179pubmed:affiliationInstitute of Cytology RAS, St. Petersburg.lld:pubmed
pubmed-article:11534179pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:11534179pubmed:publicationTypeEnglish Abstractlld:pubmed