| pubmed-article:11526037 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11526037 | lifeskim:mentions | umls-concept:C0003232 | lld:lifeskim |
| pubmed-article:11526037 | lifeskim:mentions | umls-concept:C0317964 | lld:lifeskim |
| pubmed-article:11526037 | lifeskim:mentions | umls-concept:C0699040 | lld:lifeskim |
| pubmed-article:11526037 | lifeskim:mentions | umls-concept:C0038884 | lld:lifeskim |
| pubmed-article:11526037 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:11526037 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:11526037 | lifeskim:mentions | umls-concept:C2917605 | lld:lifeskim |
| pubmed-article:11526037 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:11526037 | pubmed:dateCreated | 2001-8-29 | lld:pubmed |
| pubmed-article:11526037 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11526037 | pubmed:abstractText | The root-associated biological control bacterium Pseudomonas aureofaciens 30-84 produces a range of exoproducts, including protease and phenazines. Phenazine antibiotic biosynthesis by phzXYFABCD is regulated in part by the PhzR-PhzI quorum-sensing system. Mutants defective in phzR or phzI produce very low levels of phenazines but wild-type levels of exoprotease. In the present study, a second genomic region of strain 30-84 was identified that, when present in trans, increased beta-galactosidase activity in a genomic phzB::lacZ reporter and partially restored phenazine production to a phzR mutant. Sequence analysis identified two adjacent genes, csaR and csaI, that encode members of the LuxR-LuxI family of regulatory proteins. No putative promoter region is present upstream of the csaI start codon and no lux box-like element was found in either the csaR promoter or the 30-bp intergenic region between csaR and csaI. Both the PhzR-PhzI and CsaR-CsaI systems are regulated by the GacS-GacA two-component regulatory system. In contrast to the multicopy effects of csaR and csaI in trans, a genomic csaR mutant (30-84R2) and a csaI mutant (30-84I2) did not exhibit altered phenazine production in vitro or in situ, indicating that the CsaR-CsaI system is not involved in phenazine regulation in strain 30-84. Both mutants also produced wild-type levels of protease. However, disruption of both csaI and phzI or both csaR and phzR eliminated both phenazine and protease production completely. Thus, the two quorum-sensing systems do not interact for phenazine regulation but do interact for protease regulation. Additionally, the CsaI N-acylhomoserine lactone (AHL) signal was not recognized by the phenazine AHL reporter 30-84I/Z but was recognized by the AHL reporters Chromobacterium violaceum CV026 and Agrobacterium tumefaciens A136(pCF240). Inactivation of csaR resulted in a smooth mucoid colony phenotype and formation of cell aggregates in broth, suggesting that CsaR is involved in regulating biosynthesis of cell surface components. Strain 30-84I/I2 exhibited mucoid colony and clumping phenotypes similar to those of 30-84R2. Both phenotypes were reversed by complementation with csaR-csaI or by the addition of the CsaI AHL signal. Both quorum-sensing systems play a role in colonization by strain 30-84. Whereas loss of PhzR resulted in a 6.6-fold decrease in colonization by strain 30-84 on wheat roots in natural soil, a phzR csaR double mutant resulted in a 47-fold decrease. These data suggest that gene(s) regulated by the CsaR-CsaI system also plays a role in the rhizosphere competence of P. aureofaciens 30-84. | lld:pubmed |
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| pubmed-article:11526037 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11526037 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11526037 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:11526037 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11526037 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:11526037 | pubmed:issn | 0099-2240 | lld:pubmed |
| pubmed-article:11526037 | pubmed:author | pubmed-author:ZhangZZ | lld:pubmed |
| pubmed-article:11526037 | pubmed:author | pubmed-author:PiersonL... | lld:pubmed |
| pubmed-article:11526037 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11526037 | pubmed:volume | 67 | lld:pubmed |
| pubmed-article:11526037 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11526037 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11526037 | pubmed:pagination | 4305-15 | lld:pubmed |
| pubmed-article:11526037 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:11526037 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11526037 | pubmed:articleTitle | A second quorum-sensing system regulates cell surface properties but not phenazine antibiotic production in Pseudomonas aureofaciens. | lld:pubmed |
| pubmed-article:11526037 | pubmed:affiliation | Department of Plant Pathology, University of Arizona, Tucson, Arizona 85721, USA. | lld:pubmed |
| pubmed-article:11526037 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11526037 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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