| pubmed-article:11516485 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11516485 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:11516485 | lifeskim:mentions | umls-concept:C0003320 | lld:lifeskim |
| pubmed-article:11516485 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:11516485 | lifeskim:mentions | umls-concept:C1999216 | lld:lifeskim |
| pubmed-article:11516485 | lifeskim:mentions | umls-concept:C0162524 | lld:lifeskim |
| pubmed-article:11516485 | lifeskim:mentions | umls-concept:C2603343 | lld:lifeskim |
| pubmed-article:11516485 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:11516485 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:11516485 | pubmed:dateCreated | 2001-8-22 | lld:pubmed |
| pubmed-article:11516485 | pubmed:abstractText | A protocol for chemiluminescence detection of hantaviral antigens in infected cell foci is described. This focus detection is based on the conversion of a substrate into a luminescent product by peroxidase-antibody conjugates; the emitted light of infected cell foci can easily be recorded by autoradiography or video imaging providing a hard copy for documentation. The main advantage of this method as compared to conventional immunochemical staining is a higher detection sensitivity due to the inherent magnification effect of luminescence causing an obvious boost in focal image and intensity. This enables reduction of (i) incubation time of virus-infected cells and (ii) amount of needed antibody for antigen detection in foci. This method is applied to a chemiluminescence focus reduction assay for the serotyping of hantavirus-specific neutralising antibodies in infected persons and for the determination of activity of antiviral agents against hantavirus. | lld:pubmed |
| pubmed-article:11516485 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11516485 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11516485 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11516485 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11516485 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:11516485 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11516485 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11516485 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:11516485 | pubmed:issn | 0166-0934 | lld:pubmed |
| pubmed-article:11516485 | pubmed:author | pubmed-author:KrügerD HDH | lld:pubmed |
| pubmed-article:11516485 | pubmed:author | pubmed-author:UlrichRR | lld:pubmed |
| pubmed-article:11516485 | pubmed:author | pubmed-author:NeytsJJ | lld:pubmed |
| pubmed-article:11516485 | pubmed:author | pubmed-author:LundkvistAA | lld:pubmed |
| pubmed-article:11516485 | pubmed:author | pubmed-author:HeiderHH | lld:pubmed |
| pubmed-article:11516485 | pubmed:author | pubmed-author:PriemerCC | lld:pubmed |
| pubmed-article:11516485 | pubmed:author | pubmed-author:ZiajaBB | lld:pubmed |
| pubmed-article:11516485 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11516485 | pubmed:volume | 96 | lld:pubmed |
| pubmed-article:11516485 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11516485 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11516485 | pubmed:pagination | 17-23 | lld:pubmed |
| pubmed-article:11516485 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:11516485 | pubmed:meshHeading | pubmed-meshheading:11516485... | lld:pubmed |
| pubmed-article:11516485 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11516485 | pubmed:articleTitle | A chemiluminescence detection method of hantaviral antigens in neutralisation assays and inhibitor studies. | lld:pubmed |
| pubmed-article:11516485 | pubmed:affiliation | Institut für Virologie, Universitätsklinikum Charité, Humboldt-Universität, D-10098 Berlin, Germany. | lld:pubmed |
| pubmed-article:11516485 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11516485 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:11516485 | pubmed:publicationType | Evaluation Studies | lld:pubmed |
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