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pubmed-article:11506929pubmed:abstractTextEighty-one isolates presumptively identified as Pasteurella multocida from a variety of diseases in animals in Zimbabwe were subjected to biochemical characterization, capsular typing and RAPD analysis. The majority of isolates (over 80%) were assigned into named taxa and were predominantly P. multocida subsp. multocida and P. multocida subsp. septica, whilst the remainder were unassigned. Serogroup A was predominant among the three capsular types (A, B and D) of P. multocida detected. Three main RAPD clusters and three subclusters were observed among the majority of isolates (93.8%), whilst the remainder was found to be weakly related. Nine different groups of strains with similar RAPD profiles (100% similarity) were also observed. The reference strain of capsular serogroup F clustered with the reference strain of P. multocida subsp. septica, whilst all other serogroups clustered with reference strains of subsp. multocida and gallicida. Notably, serogroups A and D were observed to be closely related to the reference strain of subsp. multocida. The relationship between biotype, capsular type, host origin and disease manifestation was not clear-cut. However, most pig isolates of subsp. multocida clustered together as did most cattle isolates of subsp. multocida. RAPD tended to separate subsp. multocida from septica.lld:pubmed
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pubmed-article:11506929pubmed:authorpubmed-author:ChristensenHHlld:pubmed
pubmed-article:11506929pubmed:authorpubmed-author:MohanKKlld:pubmed
pubmed-article:11506929pubmed:authorpubmed-author:OlsenJ EJElld:pubmed
pubmed-article:11506929pubmed:authorpubmed-author:DzivaFFlld:pubmed
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pubmed-article:11506929pubmed:pagination361-72lld:pubmed
pubmed-article:11506929pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:11506929pubmed:year2001lld:pubmed
pubmed-article:11506929pubmed:articleTitleRandom amplification of polymorphic DNA and phenotypic typing of Zimbabwean isolates of Pasteurella multocida.lld:pubmed
pubmed-article:11506929pubmed:affiliationFaculty of Veterinary Science, University of Zimbabwe, P.O. Box MP167, Mount Pleasant, Harare, Zimbabwe. fdziva@compcentre.uz.ac.zwlld:pubmed
pubmed-article:11506929pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:11506929pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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