Linked Life Data

11473389

Source:http://linkedlifedata.com/resource/pubmed/id/11473389

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:11473389rdf:typepubmed:Citationlld:pubmed
pubmed-article:11473389lifeskim:mentionsumls-concept:C0035286lld:lifeskim
pubmed-article:11473389lifeskim:mentionsumls-concept:C0022877lld:lifeskim
pubmed-article:11473389lifeskim:mentionsumls-concept:C0806140lld:lifeskim
pubmed-article:11473389lifeskim:mentionsumls-concept:C1707927lld:lifeskim
pubmed-article:11473389pubmed:issue1lld:pubmed
pubmed-article:11473389pubmed:dateCreated2001-7-26lld:pubmed
pubmed-article:11473389pubmed:abstractTextThis laboratory previously described a single-laser flow cytometric method, which effectively resolves micronucleated erythrocyte populations in rodent peripheral blood samples. Even so, the rarity and variable size of micronuclei make it difficult to configure instrument settings consistently and define analysis regions rationally to enumerate the cell populations of interest. Murine erythrocytes from animals infected with the malaria parasite Plasmodium berghei contain a high prevalence of erythrocytes with a uniform DNA content. This biological model for micronucleated erythrocytes offers a means by which the micronucleus analysis regions can be rationally defined, and a means for controlling interexperimental variation. The experiments described herein were performed to extend these studies by testing whether malaria-infected erythrocytes could also be used to enhance the transferability of the method, as well as control intra- and interlaboratory variation. For these studies, blood samples from mice infected with malaria, or treated with vehicle or the clastogen methyl methanesulfonate, were fixed and shipped to collaborating laboratories for analysis. After configuring instrumentation parameters and guiding the position of analysis regions with the malaria-infected blood samples, micronucleated reticulocyte frequencies were measured (20,000 reticulocytes per sample). To evaluate both intra- and interlaboratory variation, five replicates were analyzed per day, and these analyses were repeated on up to five separate days. The data of 14 laboratories presented herein indicate that transferability of this flow cytometric technique is high when instrumentation is guided by the biological standard Plasmodium berghei.lld:pubmed
pubmed-article:11473389pubmed:granthttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11473389pubmed:languageenglld:pubmed
pubmed-article:11473389pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11473389pubmed:citationSubsetIMlld:pubmed
pubmed-article:11473389pubmed:statusMEDLINElld:pubmed
pubmed-article:11473389pubmed:issn0893-6692lld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:SandelinKKlld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:BroudD DDDlld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:AaronC SCSlld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:WeaverJ LJLlld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:McLeanJJlld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:PearsonAAlld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:KwanyuenPPlld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:RodgersBBlld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:HallN ENElld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:FergusonL RLRlld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:GatehouseD...lld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:JohnsonC WCWlld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:ZhaiYYlld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:HewishD RDRlld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:SlowikowskaM...lld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:BolcsfoldiGGlld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:HarbachP RPRlld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:SiennickaJJlld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:MarshallI CIClld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:FenechMMlld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:VanparysPPlld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:LynchA MAMlld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:MajeskaJ BJBlld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:HynesG MGMlld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:Van HoofVVlld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:DoughtyLLlld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:LenarczykMMlld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:TometskoC RCRlld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:DertingerS...lld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:TorousD KDKlld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:McNameeJ PJPlld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:ParenteauMMlld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:CederbrantKKlld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:DiehlM SMSlld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:Illi-LoveA...lld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:TarcaJ PJPlld:pubmed
pubmed-article:11473389pubmed:authorpubmed-author:LitwinskaBBlld:pubmed
pubmed-article:11473389pubmed:copyrightInfoCopyright 2001 Wiley-Liss, Inc.lld:pubmed
pubmed-article:11473389pubmed:issnTypePrintlld:pubmed
pubmed-article:11473389pubmed:volume38lld:pubmed
pubmed-article:11473389pubmed:ownerNLMlld:pubmed
pubmed-article:11473389pubmed:authorsCompleteYlld:pubmed
pubmed-article:11473389pubmed:pagination59-68lld:pubmed
pubmed-article:11473389pubmed:dateRevised2007-11-14lld:pubmed
pubmed-article:11473389pubmed:meshHeadingpubmed-meshheading:11473389...lld:pubmed
pubmed-article:11473389pubmed:meshHeadingpubmed-meshheading:11473389...lld:pubmed
pubmed-article:11473389pubmed:meshHeadingpubmed-meshheading:11473389...lld:pubmed
pubmed-article:11473389pubmed:meshHeadingpubmed-meshheading:11473389...lld:pubmed
pubmed-article:11473389pubmed:meshHeadingpubmed-meshheading:11473389...lld:pubmed
pubmed-article:11473389pubmed:meshHeadingpubmed-meshheading:11473389...lld:pubmed
pubmed-article:11473389pubmed:meshHeadingpubmed-meshheading:11473389...lld:pubmed
pubmed-article:11473389pubmed:meshHeadingpubmed-meshheading:11473389...lld:pubmed
pubmed-article:11473389pubmed:meshHeadingpubmed-meshheading:11473389...lld:pubmed
pubmed-article:11473389pubmed:meshHeadingpubmed-meshheading:11473389...lld:pubmed
pubmed-article:11473389pubmed:year2001lld:pubmed
pubmed-article:11473389pubmed:articleTitleFlow cytometric enumeration of micronucleated reticulocytes: high transferability among 14 laboratories.lld:pubmed
pubmed-article:11473389pubmed:affiliationLitron Laboratories, Rochester, New York 14620, USA. dtorous@litronlabs.comlld:pubmed
pubmed-article:11473389pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:11473389pubmed:publicationTypeComparative Studylld:pubmed
pubmed-article:11473389pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11473389lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11473389lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11473389lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11473389lld:pubmed