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pubmed-article:11457912pubmed:dateCreated2001-7-17lld:pubmed
pubmed-article:11457912pubmed:abstractTextExperiments were conducted to investigate the effect of S nutrition and availability on the forms of S and N in the endosperm cavity and endosperm of wheat, and on the capacity of the endosperm to utilize those compounds for the synthesis of proteins. Plants were grown in solution culture with 2 mM N and either 200 microM S (high-S) or 50 microM S (low-S) and all nutrients were withdrawn at various times from booting until 8 d post-anthesis. Sulphate was the major form of soluble S in the endosperm cavity and endosperm of high-S plants during the time of rapid grain development. By contrast, glutathione (GSH) was the major form of soluble S in the endosperm cavity and in the endosperm in low-S plants. Crude extracts of endosperm tissue from both high-S and low-S plants supported (i) the hydrolysis of GSH to gamma-glutamyl cysteine and glycine, and of gamma-glutamyl cysteine to glutamate and cysteine, and (ii) sulphate-dependent PPi-ATP exchange and the sulphydration of O-acetylserine catalysed by ATP sulphurylase and cysteine synthase, respectively. High-S nutrition enhanced the in vitro rates of ATP sulphurylase and cysteine synthase.lld:pubmed
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pubmed-article:11457912pubmed:authorpubmed-author:AndersonJ WJWlld:pubmed
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pubmed-article:11457912pubmed:pagination1519-26lld:pubmed
pubmed-article:11457912pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:11457912pubmed:year2001lld:pubmed
pubmed-article:11457912pubmed:articleTitleSulphur nutrition affects delivery and metabolism of S in developing endosperms of wheat.lld:pubmed
pubmed-article:11457912pubmed:affiliationSchool of Botany, La Trobe University, Bundoora 3083, Victoria, Australia. melissa.fitzgerald@agric.nsw.gov.aulld:pubmed
pubmed-article:11457912pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:11457912pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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