| pubmed-article:11445591 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11445591 | lifeskim:mentions | umls-concept:C1333663 | lld:lifeskim |
| pubmed-article:11445591 | lifeskim:mentions | umls-concept:C1530719 | lld:lifeskim |
| pubmed-article:11445591 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:11445591 | lifeskim:mentions | umls-concept:C0080347 | lld:lifeskim |
| pubmed-article:11445591 | lifeskim:mentions | umls-concept:C2827370 | lld:lifeskim |
| pubmed-article:11445591 | lifeskim:mentions | umls-concept:C2239482 | lld:lifeskim |
| pubmed-article:11445591 | lifeskim:mentions | umls-concept:C1514562 | lld:lifeskim |
| pubmed-article:11445591 | lifeskim:mentions | umls-concept:C1145667 | lld:lifeskim |
| pubmed-article:11445591 | pubmed:issue | 38 | lld:pubmed |
| pubmed-article:11445591 | pubmed:dateCreated | 2001-9-17 | lld:pubmed |
| pubmed-article:11445591 | pubmed:abstractText | The mammalian transcription factor GATA-1 is required for normal erythroid and megakaryocytic development. GATA-1 contains two zinc fingers, the C-terminal finger, which is known to bind (A/T)GATA(A/G) motifs in DNA and the N-finger, which is important for interacting with co-regulatory proteins such as Friend of GATA (FOG). We now show that, like the C-finger, the N-finger of GATA-1 is also capable of binding DNA but recognizes distinct sequences with the core GATC. We demonstrate that the GATA-1 N-finger can bind these sequences in vitro and that in cellular assays, GATA-1 can activate promoters containing GATC motifs. Experiments with mutant GATA-1 proteins confirm the importance of the N-finger, as the C-finger is not required for transactivation from GATC sites. Recently four naturally occurring mutations in GATA-1 have been shown to be associated with familial blood disorders. These mutations all map to the N-finger domain. We have investigated the effect of these mutations on the recognition of GATC sites by the N-finger and show that one mutation R216Q abolishes DNA binding, whereas the others have only minor effects. | lld:pubmed |
| pubmed-article:11445591 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11445591 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11445591 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:11445591 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11445591 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:11445591 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:11445591 | pubmed:author | pubmed-author:MackayJJ | lld:pubmed |
| pubmed-article:11445591 | pubmed:author | pubmed-author:NewtonAA | lld:pubmed |
| pubmed-article:11445591 | pubmed:author | pubmed-author:CrossleyMM | lld:pubmed |
| pubmed-article:11445591 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11445591 | pubmed:day | 21 | lld:pubmed |
| pubmed-article:11445591 | pubmed:volume | 276 | lld:pubmed |
| pubmed-article:11445591 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11445591 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11445591 | pubmed:pagination | 35794-801 | lld:pubmed |
| pubmed-article:11445591 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:11445591 | pubmed:meshHeading | pubmed-meshheading:11445591... | lld:pubmed |
| pubmed-article:11445591 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11445591 | pubmed:articleTitle | The N-terminal zinc finger of the erythroid transcription factor GATA-1 binds GATC motifs in DNA. | lld:pubmed |
| pubmed-article:11445591 | pubmed:affiliation | Department of Biochemistry, G08, University of Sydney, New South Wales, Australia 2006. | lld:pubmed |
| pubmed-article:11445591 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11445591 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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