| pubmed-article:11434458 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11434458 | lifeskim:mentions | umls-concept:C0040329 | lld:lifeskim |
| pubmed-article:11434458 | lifeskim:mentions | umls-concept:C0007635 | lld:lifeskim |
| pubmed-article:11434458 | lifeskim:mentions | umls-concept:C0001246 | lld:lifeskim |
| pubmed-article:11434458 | lifeskim:mentions | umls-concept:C0040674 | lld:lifeskim |
| pubmed-article:11434458 | lifeskim:mentions | umls-concept:C0449774 | lld:lifeskim |
| pubmed-article:11434458 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:11434458 | pubmed:dateCreated | 2001-7-3 | lld:pubmed |
| pubmed-article:11434458 | pubmed:abstractText | We investigated physical distances and directions of transposition of the maize transposable element Ac in tobacco cultured cells. We introduced a T-DNA construct that carried a non-autonomous derivative of Ac (designated dAc-I-RS) that included sites for cleavage by restriction endonuclease MluI. Another cleavage site was also introduced into the T-DNA region outside of the dAc-I-RS transposable element. The tobacco cultured cell line BY-2 was transformed with the T-DNA and several transformed lines that had a single copy of the T-DNA at a different chromosomal location were isolated. These lines were co-cultured with Agrobacterium tumefaciens cells that carried a cDNA for the Ac transposase gene under the control of various promoters. Sublines of cultured cells in which dAc-I-RS had been transposed, were isolated. The genomic DNAs of these sublines were isolated and digested with MluI. Sizes of DNA segments generated by digestion were determined by pulse-field gel electrophoresis. Our results showed that 20 to 70% of transposition events had occurred within several hundreds kilo-base pairs (kb) on the same chromosome. These results demonstrate that the Ac-Ds element preferentially transposed to regions near the original site in a tobacco chromosome. In addition, the present results are an example of asymmetric transposition as demonstrated by the distance of transposition on the chromosome. | lld:pubmed |
| pubmed-article:11434458 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11434458 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11434458 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11434458 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11434458 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11434458 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:11434458 | pubmed:issn | 1341-7568 | lld:pubmed |
| pubmed-article:11434458 | pubmed:author | pubmed-author:IshikawaTT | lld:pubmed |
| pubmed-article:11434458 | pubmed:author | pubmed-author:MachidaYY | lld:pubmed |
| pubmed-article:11434458 | pubmed:author | pubmed-author:TorikaiSS | lld:pubmed |
| pubmed-article:11434458 | pubmed:author | pubmed-author:MachidaCC | lld:pubmed |
| pubmed-article:11434458 | pubmed:author | pubmed-author:OnouchiHH | lld:pubmed |
| pubmed-article:11434458 | pubmed:author | pubmed-author:SemiartiEE | lld:pubmed |
| pubmed-article:11434458 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11434458 | pubmed:volume | 76 | lld:pubmed |
| pubmed-article:11434458 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11434458 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11434458 | pubmed:pagination | 131-9 | lld:pubmed |
| pubmed-article:11434458 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
| pubmed-article:11434458 | pubmed:meshHeading | pubmed-meshheading:11434458... | lld:pubmed |
| pubmed-article:11434458 | pubmed:meshHeading | pubmed-meshheading:11434458... | lld:pubmed |
| pubmed-article:11434458 | pubmed:meshHeading | pubmed-meshheading:11434458... | lld:pubmed |
| pubmed-article:11434458 | pubmed:meshHeading | pubmed-meshheading:11434458... | lld:pubmed |
| pubmed-article:11434458 | pubmed:meshHeading | pubmed-meshheading:11434458... | lld:pubmed |
| pubmed-article:11434458 | pubmed:meshHeading | pubmed-meshheading:11434458... | lld:pubmed |
| pubmed-article:11434458 | pubmed:meshHeading | pubmed-meshheading:11434458... | lld:pubmed |
| pubmed-article:11434458 | pubmed:meshHeading | pubmed-meshheading:11434458... | lld:pubmed |
| pubmed-article:11434458 | pubmed:meshHeading | pubmed-meshheading:11434458... | lld:pubmed |
| pubmed-article:11434458 | pubmed:meshHeading | pubmed-meshheading:11434458... | lld:pubmed |
| pubmed-article:11434458 | pubmed:meshHeading | pubmed-meshheading:11434458... | lld:pubmed |
| pubmed-article:11434458 | pubmed:meshHeading | pubmed-meshheading:11434458... | lld:pubmed |
| pubmed-article:11434458 | pubmed:meshHeading | pubmed-meshheading:11434458... | lld:pubmed |
| pubmed-article:11434458 | pubmed:meshHeading | pubmed-meshheading:11434458... | lld:pubmed |
| pubmed-article:11434458 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11434458 | pubmed:articleTitle | The transposition pattern of the Ac element in tobacco cultured cells. | lld:pubmed |
| pubmed-article:11434458 | pubmed:affiliation | Division of Biological Science, Graduate School of Science, Nagoya University, Japan. | lld:pubmed |
| pubmed-article:11434458 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11434458 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11434458 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11434458 | lld:pubmed |
