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pubmed-article:11422376pubmed:abstractTextMitochondrial chaperonins are necessary for the folding of newly imported and stress-denatured mitochondrial proteins. The goal of this study was to investigate the structure and function of the mammalian mitochondrial chaperonin system. We present evidence that the 60 kDa chaperonin (mt-cpn60) exists in solution in dynamic equilibrium between monomers, heptameric single rings and double-ringed tetradecamers. In the presence of ATP and the 10 kDa cochaperonin (mt-cpn10), the formation of a double ring is favored. ADP at very high concentrations does not inhibit malate dehydrogenase refolding or ATP hydrolysis by mt-cpn60 in the presence of mt-cpn10. We propose that the cis (mt-cpn60)14.nucleotide.(mt-cpn10)7 complex is not a stable species and does not bind ADP effectively at its trans binding site.lld:pubmed
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pubmed-article:11422376pubmed:articleTitleThe effect of nucleotides and mitochondrial chaperonin 10 on the structure and chaperone activity of mitochondrial chaperonin 60.lld:pubmed
pubmed-article:11422376pubmed:affiliationDepartment of Biochemistry, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Israel.lld:pubmed
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pubmed-article:11422376pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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