| pubmed-article:11412113 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11412113 | lifeskim:mentions | umls-concept:C0021760 | lld:lifeskim |
| pubmed-article:11412113 | lifeskim:mentions | umls-concept:C0524637 | lld:lifeskim |
| pubmed-article:11412113 | lifeskim:mentions | umls-concept:C1325331 | lld:lifeskim |
| pubmed-article:11412113 | lifeskim:mentions | umls-concept:C1704241 | lld:lifeskim |
| pubmed-article:11412113 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:11412113 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:11412113 | pubmed:issue | 25 | lld:pubmed |
| pubmed-article:11412113 | pubmed:dateCreated | 2001-6-19 | lld:pubmed |
| pubmed-article:11412113 | pubmed:abstractText | Gp130 is a shared signal-transducing receptor for a family of four-helix cytokines, of which interleukin-6 is a prototypic member. IL-6-type cytokines activate gp130 to elicit downstream intracellular JAK/STAT signaling cascades through formation of hetero-oligomeric receptor complexes. Interleukin-6 must first complex with its specific alpha-receptor (Ralpha) in order to bind and activate gp130. We have dissected the extracellular activation pathway of human gp130 by human IL-6 through reconstitution of soluble complexes representing intermediate and final states in the hierarchical assembly of the IL-6/IL-6Ralpha/gp130 signaling complex. To isolate these hetero-complexes, we have applied a protein engineering strategy of covalently linking IL-6 to its Ralpha, which results in a "hyperactive" single-chain complex (hyper-IL-6) which we express in both Escherichia coli and insect cells. We have determined that IL-6/IL-Ralpha and the cytokine-binding homology region (CHR) of gp130 (D2D3) form a stable trimolecular "recognition" complex (trimer) consisting of 1IL-6,1 IL-6Ralpha, and 1 gp130-CHR. Addition of the N-terminal (D1) Ig-like domain (IGD) of gp130 to the CHR results in a transition to a hexameric "activation" complex containing 2 IL-6, 2IL-6Ralpha, and 2 gp130. These results clearly demonstrate that the recognition and activation complexes are disparate hetero-oligomeric molecular species linked by the recruitment of the gp130 IGD by the unique site III epitope present on all gp130-class cytokines. The results of these studies are relevant to other members of the IL-6 family of gp130-cytokines and address a longstanding question concerning the respective roles of the gp130 CHR and IGD in assembly of the active signaling oligomer. | lld:pubmed |
| pubmed-article:11412113 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11412113 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11412113 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11412113 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11412113 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11412113 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11412113 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11412113 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11412113 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11412113 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11412113 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11412113 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11412113 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11412113 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11412113 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11412113 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:11412113 | pubmed:issn | 0006-2960 | lld:pubmed |
| pubmed-article:11412113 | pubmed:author | pubmed-author:FoxK WKW | lld:pubmed |
| pubmed-article:11412113 | pubmed:author | pubmed-author:Rose-JohnSS | lld:pubmed |
| pubmed-article:11412113 | pubmed:author | pubmed-author:GarciaK CKC | lld:pubmed |
| pubmed-article:11412113 | pubmed:author | pubmed-author:ChotoRR | lld:pubmed |
| pubmed-article:11412113 | pubmed:author | pubmed-author:Nguyen... | lld:pubmed |
| pubmed-article:11412113 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11412113 | pubmed:day | 26 | lld:pubmed |
| pubmed-article:11412113 | pubmed:volume | 40 | lld:pubmed |
| pubmed-article:11412113 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11412113 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11412113 | pubmed:pagination | 7593-603 | lld:pubmed |
| pubmed-article:11412113 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:11412113 | pubmed:meshHeading | pubmed-meshheading:11412113... | lld:pubmed |
| pubmed-article:11412113 | pubmed:meshHeading | pubmed-meshheading:11412113... | lld:pubmed |
| pubmed-article:11412113 | pubmed:meshHeading | pubmed-meshheading:11412113... | lld:pubmed |
| pubmed-article:11412113 | pubmed:meshHeading | pubmed-meshheading:11412113... | lld:pubmed |
| pubmed-article:11412113 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11412113 | pubmed:articleTitle | In vitro reconstitution of recognition and activation complexes between interleukin-6 and gp130. | lld:pubmed |
| pubmed-article:11412113 | pubmed:affiliation | Departments of Microbiology & Immunology, and Structural Biology, Stanford University School of Medicine, Fairchild D319, 299 Campus Drive, Stanford, California 94305-5124, USA. | lld:pubmed |
| pubmed-article:11412113 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11412113 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:11412113 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11412113 | lld:pubmed |
