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pubmed-article:11396959pubmed:abstractTextDegradation of bisphenol A (BPA), an endocrine-disrupting chemical, was studied with a purified laccase from the basidiomycete Trametes villosa. SDS--polyacrylamide gel electrophoresis of the purified laccase gave one single band with a mobility corresponding to MW 65 kDa. The absorption spectrum showed the characteristics of a blue copper protein with a maximum peak at 600 nm. HPLC analysis revealed that 2.2 micromol BPA were degraded by incubation with 1.5 units of the purified laccase in a total volume of 1.0 ml at pH 6.0 and 60 degrees C for 3 h. The enzyme reaction proceeded rapidly without requirement of mediators for the electron transfer. Isolation and identification of several reaction products are in progress, in which one product was identified as 4-isopropenylphenol by a gas chromatography--mass spectrophotometer.lld:pubmed
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pubmed-article:11396959pubmed:copyrightInfoCopyright 2001 Academic Press.lld:pubmed
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pubmed-article:11396959pubmed:pagination704-6lld:pubmed
pubmed-article:11396959pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:11396959pubmed:articleTitleDegradation of bisphenol A by purified laccase from Trametes villosa.lld:pubmed
pubmed-article:11396959pubmed:affiliationApplied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, 9-1, Bunkyou 3-Chome, Fukui-shi 910-8507, Japan.lld:pubmed
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