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pubmed-article:11393295pubmed:abstractTextThis report describes a method for the isolation of nucleic acid from a suspension of matured virus. Nucleic acid (DNA) was isolated from a prototype strain of adenovirus type 7 and a clinical isolate of adenovirus type 7. Instead of the usual method of ultracentifugation, a filtration method was applied to concentrate the virus rapidly and nucleic acid was then isolated by a standard phenol/chloroform/isoamyl-alcohol extraction procedure. The DNA was found to be sufficiently purified to generate a reproducible restriction endonuclease digestion pattern. The clinical isolate of adenovirus type 7 revealed loss of restriction site for the endonuclease HindIII when compared with the prototype strain.lld:pubmed
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pubmed-article:11393295pubmed:authorpubmed-author:PoddarS KSKlld:pubmed
pubmed-article:11393295pubmed:authorpubmed-author:GrayG CGClld:pubmed
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pubmed-article:11393295pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:11393295pubmed:articleTitleA modified rapid method of nucleic acid isolation from suspension of matured virus: applied in restriction analysis of DNA from an adenovirus prototype strain and a patient isolate.lld:pubmed
pubmed-article:11393295pubmed:affiliationNaval Health Research Center, DoD Center for Deployment Health Research, San Diego, CA 92186-5122, USA.lld:pubmed
pubmed-article:11393295pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:11393295pubmed:publicationTypeComparative Studylld:pubmed
pubmed-article:11393295pubmed:publicationTypeResearch Support, U.S. Gov't, Non-P.H.S.lld:pubmed
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