| pubmed-article:11390681 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11390681 | lifeskim:mentions | umls-concept:C0319506 | lld:lifeskim |
| pubmed-article:11390681 | lifeskim:mentions | umls-concept:C1843926 | lld:lifeskim |
| pubmed-article:11390681 | lifeskim:mentions | umls-concept:C0597298 | lld:lifeskim |
| pubmed-article:11390681 | lifeskim:mentions | umls-concept:C0332281 | lld:lifeskim |
| pubmed-article:11390681 | lifeskim:mentions | umls-concept:C0065558 | lld:lifeskim |
| pubmed-article:11390681 | lifeskim:mentions | umls-concept:C2603343 | lld:lifeskim |
| pubmed-article:11390681 | pubmed:issue | Pt 6 | lld:pubmed |
| pubmed-article:11390681 | pubmed:dateCreated | 2001-6-6 | lld:pubmed |
| pubmed-article:11390681 | pubmed:abstractText | The oleaginous fungus Mucor circinelloides possesses at least six isoforms of malic enzyme (EC 1.1.1.40), a key lipogenic enzyme in filamentous fungi. These isoforms were detected using a specific stain for activity after native PAGE of cell extracts. Only one isoform (isoform IV) was associated with lipid accumulation, appearing only after N-exhaustion from the medium (which is a pre-requisite for lipid accumulation) in glucose-growing cells. Isoforms I, II, V and VI were involved in anaerobic growth and only appeared under O(2)-limited conditions. Isoform III appeared to be constitutive and was formed under conditions of active (balanced) growth and is therefore thought to play a crucial role in basic metabolism. Growth on acetate increased the amount of cell lipid (from 25-27% in glucose-grown cells to 37-38% in acetate-grown cells) accumulated by M. circinelloides and this was associated with the appearance of isoform IV of malic enzyme prior to N-exhaustion in these cultures. Amino acid sequence analysis of isoforms III and IV suggests that these two malic enzymes may be encoded by a single gene and that isoform IV is formed from isoform III by post-translational modification initiated by either N-limitation (when glucose was the carbon source) or growth on acetate as the sole carbon source. | lld:pubmed |
| pubmed-article:11390681 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11390681 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11390681 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11390681 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11390681 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11390681 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11390681 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11390681 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11390681 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11390681 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11390681 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11390681 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:11390681 | pubmed:issn | 1350-0872 | lld:pubmed |
| pubmed-article:11390681 | pubmed:author | pubmed-author:LUMM | lld:pubmed |
| pubmed-article:11390681 | pubmed:author | pubmed-author:RatledgeCC | lld:pubmed |
| pubmed-article:11390681 | pubmed:author | pubmed-author:SongYY | lld:pubmed |
| pubmed-article:11390681 | pubmed:author | pubmed-author:WynnJ PJP | lld:pubmed |
| pubmed-article:11390681 | pubmed:author | pubmed-author:GranthamDD | lld:pubmed |
| pubmed-article:11390681 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11390681 | pubmed:volume | 147 | lld:pubmed |
| pubmed-article:11390681 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11390681 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11390681 | pubmed:pagination | 1507-15 | lld:pubmed |
| pubmed-article:11390681 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:11390681 | pubmed:meshHeading | pubmed-meshheading:11390681... | lld:pubmed |
| pubmed-article:11390681 | pubmed:meshHeading | pubmed-meshheading:11390681... | lld:pubmed |
| pubmed-article:11390681 | pubmed:meshHeading | pubmed-meshheading:11390681... | lld:pubmed |
| pubmed-article:11390681 | pubmed:meshHeading | pubmed-meshheading:11390681... | lld:pubmed |
| pubmed-article:11390681 | pubmed:meshHeading | pubmed-meshheading:11390681... | lld:pubmed |
| pubmed-article:11390681 | pubmed:meshHeading | pubmed-meshheading:11390681... | lld:pubmed |
| pubmed-article:11390681 | pubmed:meshHeading | pubmed-meshheading:11390681... | lld:pubmed |
| pubmed-article:11390681 | pubmed:meshHeading | pubmed-meshheading:11390681... | lld:pubmed |
| pubmed-article:11390681 | pubmed:meshHeading | pubmed-meshheading:11390681... | lld:pubmed |
| pubmed-article:11390681 | pubmed:meshHeading | pubmed-meshheading:11390681... | lld:pubmed |
| pubmed-article:11390681 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11390681 | pubmed:articleTitle | A pre-genetic study of the isoforms of malic enzyme associated with lipid accumulation in Mucor circinelloides. | lld:pubmed |
| pubmed-article:11390681 | pubmed:affiliation | Centre for Advanced Lipid Research, Department of Biological Sciences, University of Hull, Hull HU6 7RX, UK. | lld:pubmed |
| pubmed-article:11390681 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11390681 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
