| pubmed-article:11381130 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11381130 | lifeskim:mentions | umls-concept:C2752415 | lld:lifeskim |
| pubmed-article:11381130 | lifeskim:mentions | umls-concept:C0302523 | lld:lifeskim |
| pubmed-article:11381130 | lifeskim:mentions | umls-concept:C0700325 | lld:lifeskim |
| pubmed-article:11381130 | lifeskim:mentions | umls-concept:C0332523 | lld:lifeskim |
| pubmed-article:11381130 | lifeskim:mentions | umls-concept:C0439851 | lld:lifeskim |
| pubmed-article:11381130 | lifeskim:mentions | umls-concept:C0231448 | lld:lifeskim |
| pubmed-article:11381130 | lifeskim:mentions | umls-concept:C1552596 | lld:lifeskim |
| pubmed-article:11381130 | lifeskim:mentions | umls-concept:C1947931 | lld:lifeskim |
| pubmed-article:11381130 | pubmed:issue | 12 | lld:pubmed |
| pubmed-article:11381130 | pubmed:dateCreated | 2001-6-6 | lld:pubmed |
| pubmed-article:11381130 | pubmed:abstractText | Type IV pili are thin filaments that extend from the poles of a diverse group of bacteria, enabling them to move at speeds of a few tenths of a micrometer per second. They are required for twitching motility, e.g., in Pseudomonas aeruginosa and Neisseria gonorrhoeae, and for social gliding motility in Myxococcus xanthus. Here we report direct observation of extension and retraction of type IV pili in P. aeruginosa. Cells without flagellar filaments were labeled with an amino-specific Cy3 fluorescent dye and were visualized on a quartz slide by total internal reflection microscopy. When pili were attached to a cell and their distal ends were free, they extended or retracted at rates of about 0.5 microm s(-1) (29 degrees C). They also flexed by Brownian motion, exhibiting a persistence length of about 5 microm. Frequently, the distal tip of a filament adsorbed to the substratum and the filament was pulled taut. From the absence of lateral deflections of such filaments, we estimate tensions of at least 10 pN. Occasionally, cell bodies came free and were pulled forward by pilus retraction. Thus, type IV pili are linear actuators that extend, attach at their distal tips, exert substantial force, and retract. | lld:pubmed |
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| pubmed-article:11381130 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11381130 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11381130 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11381130 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11381130 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:11381130 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:11381130 | pubmed:author | pubmed-author:BergH CHC | lld:pubmed |
| pubmed-article:11381130 | pubmed:author | pubmed-author:SkerkerJ MJM | lld:pubmed |
| pubmed-article:11381130 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11381130 | pubmed:day | 5 | lld:pubmed |
| pubmed-article:11381130 | pubmed:volume | 98 | lld:pubmed |
| pubmed-article:11381130 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11381130 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11381130 | pubmed:pagination | 6901-4 | lld:pubmed |
| pubmed-article:11381130 | pubmed:dateRevised | 2010-9-14 | lld:pubmed |
| pubmed-article:11381130 | pubmed:meshHeading | pubmed-meshheading:11381130... | lld:pubmed |
| pubmed-article:11381130 | pubmed:meshHeading | pubmed-meshheading:11381130... | lld:pubmed |
| pubmed-article:11381130 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11381130 | pubmed:articleTitle | Direct observation of extension and retraction of type IV pili. | lld:pubmed |
| pubmed-article:11381130 | pubmed:affiliation | Rowland Institute for Science, Cambridge, MA 02142, USA. | lld:pubmed |
| pubmed-article:11381130 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11381130 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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