pubmed-article:11358968 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:11358968 | lifeskim:mentions | umls-concept:C0262950 | lld:lifeskim |
pubmed-article:11358968 | lifeskim:mentions | umls-concept:C0033235 | lld:lifeskim |
pubmed-article:11358968 | lifeskim:mentions | umls-concept:C1709694 | lld:lifeskim |
pubmed-article:11358968 | lifeskim:mentions | umls-concept:C0086597 | lld:lifeskim |
pubmed-article:11358968 | lifeskim:mentions | umls-concept:C1707271 | lld:lifeskim |
pubmed-article:11358968 | pubmed:issue | 29 | lld:pubmed |
pubmed-article:11358968 | pubmed:dateCreated | 2001-7-16 | lld:pubmed |
pubmed-article:11358968 | pubmed:abstractText | The processing of the fibrillar procollagen precursors to mature collagens is an essential requirement for fibril formation. The enzymes involved in these events are known as the procollagen N and C proteinases. The latter, which cleaves the C-propeptides of the fibrillar procollagens I-III, is identical to the previously described bone morphogenetic protein-1 (BMP-1). Surprisingly, unlike the other fibrillar collagens, the processing of the C-propeptide domain of the procollagen V homotrimer was found to be mediated by furin rather than BMP-1. However, the presence of putative BMP-1 cleavage sites in the alpha1(V) C-propeptide sequence prompted us to reconsider the procollagen V C-propeptide cleavage by BMP-1. Using a recombinant system to produce substantial amounts of the proalpha1(V) homotrimer, we have previously shown that the C-propeptide is spontaneously released in the culture medium. The trimeric C-propeptide fragment, resulting from the furin cleavage, still encompassed the predicted BMP-1 cleavage sites. It was purified and tested as a substrate for BMP-1. In parallel, the release of the C-propeptide in the culture medium was inhibited by the addition of a specific furin inhibitor, allowing the re-examination of BMP-1 activity on the intact molecule. We showed that BMP-1 does cleave both substrates at one of the two predicted C-proteinase cleavage sites. Our results favor a role for PCP/BMP-1 in physiological C-terminal processing of procollagen V and imply a general mechanism for fibrillar collagen C-terminal processing. | lld:pubmed |
pubmed-article:11358968 | pubmed:language | eng | lld:pubmed |
pubmed-article:11358968 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:11358968 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:11358968 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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pubmed-article:11358968 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:11358968 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:11358968 | pubmed:month | Jul | lld:pubmed |
pubmed-article:11358968 | pubmed:issn | 0021-9258 | lld:pubmed |
pubmed-article:11358968 | pubmed:author | pubmed-author:KesslerEE | lld:pubmed |
pubmed-article:11358968 | pubmed:author | pubmed-author:RuggieroFF | lld:pubmed |
pubmed-article:11358968 | pubmed:author | pubmed-author:FichardAA | lld:pubmed |
pubmed-article:11358968 | pubmed:author | pubmed-author:BruselMM | lld:pubmed |
pubmed-article:11358968 | pubmed:author | pubmed-author:Chanut-Delala... | lld:pubmed |
pubmed-article:11358968 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:11358968 | pubmed:day | 20 | lld:pubmed |
pubmed-article:11358968 | pubmed:volume | 276 | lld:pubmed |
pubmed-article:11358968 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:11358968 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:11358968 | pubmed:pagination | 27051-7 | lld:pubmed |
pubmed-article:11358968 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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pubmed-article:11358968 | pubmed:year | 2001 | lld:pubmed |
pubmed-article:11358968 | pubmed:articleTitle | Bone morphogenetic protein-1 (BMP-1) mediates C-terminal processing of procollagen V homotrimer. | lld:pubmed |
pubmed-article:11358968 | pubmed:affiliation | Tel-Aviv University Sackler Faculty of Medicine, Goldschleger Eye Research Institute, Sheba Medical Center, Tel-Hashomer 52621, Israel. | lld:pubmed |
pubmed-article:11358968 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:11358968 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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