| pubmed-article:11334412 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11334412 | lifeskim:mentions | umls-concept:C0752063 | lld:lifeskim |
| pubmed-article:11334412 | lifeskim:mentions | umls-concept:C0021655 | lld:lifeskim |
| pubmed-article:11334412 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:11334412 | lifeskim:mentions | umls-concept:C0333516 | lld:lifeskim |
| pubmed-article:11334412 | lifeskim:mentions | umls-concept:C0071097 | lld:lifeskim |
| pubmed-article:11334412 | lifeskim:mentions | umls-concept:C0441712 | lld:lifeskim |
| pubmed-article:11334412 | lifeskim:mentions | umls-concept:C0332291 | lld:lifeskim |
| pubmed-article:11334412 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:11334412 | pubmed:dateCreated | 2001-5-3 | lld:pubmed |
| pubmed-article:11334412 | pubmed:abstractText | Tumor necrosis factor (TNF)-alpha is one of the candidate mediators of insulin resistance associated with obesity, a major risk factor for the development of type 2 diabetes. The insulin resistance induced by TNF-alpha is antagonized by thiazolidinediones (TZDs), a new class of insulin-sensitizing drugs. The aim of the current study was to dissect the mechanism whereby pioglitazone, one of the TZDs, ameliorates TNF-alpha-induced insulin resistance in 3T3-L1 adipocytes. Pioglitazone restored insulin-stimulated 2-deoxyglucose (DOG) uptake, which was reduced by TNF-alpha, with concomitant restorations in tyrosine phosphorylation and protein levels of insulin receptor (IR) and insulin receptor substrate (IRS)-1, as well as association of the p85 regulatory subunit of phosphatidylinositol (PI) 3-kinase with IRS-1 and PI 3-kinase activity. Adenovirus-mediated gene transfer of either wild-type human peroxisome proliferator-activated receptor (PPAR)-gamma2 or a mutant carrying a replacement at the consensus mitogen-activated protein kinase phosphorylation site (hPPAR-gamma2-S112A) promoted adipogenesis of 3T3-L1 fibroblasts and restored TNF-alpha-induced decrease of triglyceride in adipocytes as effectively as pioglitazone. Overexpression of the PPAR-gamma proteins in TNF-alpha-treated adipocytes restored protein levels of IR/IRS-1, but did not improve insulin-stimulated tyrosine phosphorylation of IR/IRS-1 or insulin-stimulated 2-DOG uptake. These results indicate that the ability of pioglitazone to restore insulin-stimulated tyrosine phosphorylation of IR/IRS-1, which is necessary for amelioration of TNF-alpha-induced insulin resistance, may be independent of the adipogenic activity of PPAR-gamma that regulates protein levels of IR/IRS-1. | lld:pubmed |
| pubmed-article:11334412 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11334412 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11334412 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:11334412 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11334412 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11334412 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11334412 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11334412 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11334412 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11334412 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11334412 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11334412 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11334412 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11334412 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11334412 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11334412 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11334412 | pubmed:month | May | lld:pubmed |
| pubmed-article:11334412 | pubmed:issn | 0012-1797 | lld:pubmed |
| pubmed-article:11334412 | pubmed:author | pubmed-author:KobayashiMM | lld:pubmed |
| pubmed-article:11334412 | pubmed:author | pubmed-author:UnoTT | lld:pubmed |
| pubmed-article:11334412 | pubmed:author | pubmed-author:TakataYY | lld:pubmed |
| pubmed-article:11334412 | pubmed:author | pubmed-author:IwataMM | lld:pubmed |
| pubmed-article:11334412 | pubmed:author | pubmed-author:SasaokaTT | lld:pubmed |
| pubmed-article:11334412 | pubmed:author | pubmed-author:UenoEE | lld:pubmed |
| pubmed-article:11334412 | pubmed:author | pubmed-author:HarutaTT | lld:pubmed |
| pubmed-article:11334412 | pubmed:author | pubmed-author:KawaharaJJ | lld:pubmed |
| pubmed-article:11334412 | pubmed:author | pubmed-author:IshibashiOO | lld:pubmed |
| pubmed-article:11334412 | pubmed:author | pubmed-author:TakanoAA | lld:pubmed |
| pubmed-article:11334412 | pubmed:author | pubmed-author:UsuiII | lld:pubmed |
| pubmed-article:11334412 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11334412 | pubmed:volume | 50 | lld:pubmed |
| pubmed-article:11334412 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11334412 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11334412 | pubmed:pagination | 1083-92 | lld:pubmed |
| pubmed-article:11334412 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:11334412 | pubmed:meshHeading | pubmed-meshheading:11334412... | lld:pubmed |
| pubmed-article:11334412 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11334412 | pubmed:articleTitle | Pioglitazone ameliorates tumor necrosis factor-alpha-induced insulin resistance by a mechanism independent of adipogenic activity of peroxisome proliferator--activated receptor-gamma. | lld:pubmed |
| pubmed-article:11334412 | pubmed:affiliation | First Department of Medicine, Toyama Medical and Pharmaceutical University, Japan. | lld:pubmed |
| pubmed-article:11334412 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11334412 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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