| pubmed-article:11313818 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11313818 | lifeskim:mentions | umls-concept:C0019704 | lld:lifeskim |
| pubmed-article:11313818 | lifeskim:mentions | umls-concept:C0021311 | lld:lifeskim |
| pubmed-article:11313818 | lifeskim:mentions | umls-concept:C0178719 | lld:lifeskim |
| pubmed-article:11313818 | lifeskim:mentions | umls-concept:C1883036 | lld:lifeskim |
| pubmed-article:11313818 | lifeskim:mentions | umls-concept:C0021467 | lld:lifeskim |
| pubmed-article:11313818 | lifeskim:mentions | umls-concept:C0013081 | lld:lifeskim |
| pubmed-article:11313818 | lifeskim:mentions | umls-concept:C1332823 | lld:lifeskim |
| pubmed-article:11313818 | lifeskim:mentions | umls-concept:C0021469 | lld:lifeskim |
| pubmed-article:11313818 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:11313818 | pubmed:dateCreated | 2001-4-23 | lld:pubmed |
| pubmed-article:11313818 | pubmed:abstractText | CXCR4 is the major co-receptor used by X4 strains of human immunodeficiency virus type I (HIV-1). In HIV-1-infected patients, the appearance of X4 strains (T cell line-tropic) correlates with disease progression. Since its discovery, the CXCR4 co-receptor has been a major target for different agents which block its function, such as stromal-derived factor 1alpha (SDF-1alpha) and the anti-CXCR4 monoclonal antibody, 12G5. In the present studies, the 12G5 hybridoma was used to construct a single-chain variable antibody fragment (SFv). Murine leukemia virus (MLV) and simian virus 40 (SV(40)) were utilized as delivery vehicles for the anti-CXCR4 SFv. Intracellular expression of the anti-CXCR4 SFv led to down-regulation of this critical co-receptor, as demonstrated by immunostaining. This effect significantly and specifically protected transduced cells from challenge with HIV-1, as measured by HIV-1 p24 antigen expression. Inhibition of HIV-1 replication was specific for X4 HIV-1 strains as demonstrated by MAGI assays. HeLa-CD4/betagal-CCR5 cells expressing the anti-CXCR4 SFv showed significant inhibition of infectivity by the X4 HIV-1 strain NL4-3, but not with the R5 HIV-1 strain Bal. Thus, this anti-HIV-1 molecular therapy has the potential to inhibit HIV-1 replication and virion spread. Targeting CXCR4 by intracellular immunization could be of additional benefit to certain HIV-1-infected patients on highly active antiretroviral therapy (HAART). | lld:pubmed |
| pubmed-article:11313818 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11313818 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11313818 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11313818 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11313818 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11313818 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11313818 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11313818 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11313818 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11313818 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11313818 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11313818 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:11313818 | pubmed:issn | 0969-7128 | lld:pubmed |
| pubmed-article:11313818 | pubmed:author | pubmed-author:StrayerD SDS | lld:pubmed |
| pubmed-article:11313818 | pubmed:author | pubmed-author:PomerantzR... | lld:pubmed |
| pubmed-article:11313818 | pubmed:author | pubmed-author:WeiDD | lld:pubmed |
| pubmed-article:11313818 | pubmed:author | pubmed-author:HoxieJJ | lld:pubmed |
| pubmed-article:11313818 | pubmed:author | pubmed-author:MukhtarMM | lld:pubmed |
| pubmed-article:11313818 | pubmed:author | pubmed-author:DuanL XLX | lld:pubmed |
| pubmed-article:11313818 | pubmed:author | pubmed-author:BouHamdanMM | lld:pubmed |
| pubmed-article:11313818 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11313818 | pubmed:volume | 8 | lld:pubmed |
| pubmed-article:11313818 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11313818 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11313818 | pubmed:pagination | 408-18 | lld:pubmed |
| pubmed-article:11313818 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:11313818 | pubmed:meshHeading | pubmed-meshheading:11313818... | lld:pubmed |
| pubmed-article:11313818 | pubmed:meshHeading | pubmed-meshheading:11313818... | lld:pubmed |
| pubmed-article:11313818 | pubmed:meshHeading | pubmed-meshheading:11313818... | lld:pubmed |
| pubmed-article:11313818 | pubmed:meshHeading | pubmed-meshheading:11313818... | lld:pubmed |
| pubmed-article:11313818 | pubmed:meshHeading | pubmed-meshheading:11313818... | lld:pubmed |
| pubmed-article:11313818 | pubmed:meshHeading | pubmed-meshheading:11313818... | lld:pubmed |
| pubmed-article:11313818 | pubmed:meshHeading | pubmed-meshheading:11313818... | lld:pubmed |
| pubmed-article:11313818 | pubmed:meshHeading | pubmed-meshheading:11313818... | lld:pubmed |
| pubmed-article:11313818 | pubmed:meshHeading | pubmed-meshheading:11313818... | lld:pubmed |
| pubmed-article:11313818 | pubmed:meshHeading | pubmed-meshheading:11313818... | lld:pubmed |
| pubmed-article:11313818 | pubmed:meshHeading | pubmed-meshheading:11313818... | lld:pubmed |
| pubmed-article:11313818 | pubmed:meshHeading | pubmed-meshheading:11313818... | lld:pubmed |
| pubmed-article:11313818 | pubmed:meshHeading | pubmed-meshheading:11313818... | lld:pubmed |
| pubmed-article:11313818 | pubmed:meshHeading | pubmed-meshheading:11313818... | lld:pubmed |
| pubmed-article:11313818 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11313818 | pubmed:articleTitle | Inhibition of HIV-1 infection by down-regulation of the CXCR4 co-receptor using an intracellular single chain variable fragment against CXCR4. | lld:pubmed |
| pubmed-article:11313818 | pubmed:affiliation | The Dorrance H Hamilton Laboratories, Center for Human Virology, Division of Infectious Diseases, Department of Medicine, USA. | lld:pubmed |
| pubmed-article:11313818 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11313818 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11313818 | lld:pubmed |
