| pubmed-article:11311349 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11311349 | lifeskim:mentions | umls-concept:C0019169 | lld:lifeskim |
| pubmed-article:11311349 | lifeskim:mentions | umls-concept:C0032520 | lld:lifeskim |
| pubmed-article:11311349 | lifeskim:mentions | umls-concept:C0392762 | lld:lifeskim |
| pubmed-article:11311349 | lifeskim:mentions | umls-concept:C0439831 | lld:lifeskim |
| pubmed-article:11311349 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:11311349 | pubmed:dateCreated | 2001-4-20 | lld:pubmed |
| pubmed-article:11311349 | pubmed:abstractText | Quantification of hepatitis B virus (HBV) DNA in serum is important for monitoring treatment. A rapid and cost effective alternative to the methods available currently was developed based on a real-time quantitative polymerase chain reaction (PCR) done in the LightCycler apparatus. Primers and a probe for sequences of the surface gene of HBV were designed and quantification achieved by reference to standards containing known concentrations of the target sequence. A single copy of the HBV genome could be detected if present in the reaction mixture. The quantitative range of the assay was from 4 x 10(2) to 1.3 x 10(10) surface gene copies/ml serum. Nested PCR was required for quantification in the lower part of this range (<10(5) copies). The real-time PCR and Amplicor Monitor (Roche) tests performed comparably at virus concentrations below 10(6) copies/ml. The commercial test underestimated higher concentrations of virus. | lld:pubmed |
| pubmed-article:11311349 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11311349 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11311349 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11311349 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11311349 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11311349 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11311349 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:11311349 | pubmed:issn | 0166-0934 | lld:pubmed |
| pubmed-article:11311349 | pubmed:author | pubmed-author:SaundersN ANA | lld:pubmed |
| pubmed-article:11311349 | pubmed:author | pubmed-author:DusheikoG MGM | lld:pubmed |
| pubmed-article:11311349 | pubmed:author | pubmed-author:WhalleyS ASA | lld:pubmed |
| pubmed-article:11311349 | pubmed:author | pubmed-author:BrechtbuehlKK | lld:pubmed |
| pubmed-article:11311349 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11311349 | pubmed:volume | 93 | lld:pubmed |
| pubmed-article:11311349 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11311349 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11311349 | pubmed:pagination | 105-13 | lld:pubmed |
| pubmed-article:11311349 | pubmed:dateRevised | 2004-11-17 | lld:pubmed |
| pubmed-article:11311349 | pubmed:meshHeading | pubmed-meshheading:11311349... | lld:pubmed |
| pubmed-article:11311349 | pubmed:meshHeading | pubmed-meshheading:11311349... | lld:pubmed |
| pubmed-article:11311349 | pubmed:meshHeading | pubmed-meshheading:11311349... | lld:pubmed |
| pubmed-article:11311349 | pubmed:meshHeading | pubmed-meshheading:11311349... | lld:pubmed |
| pubmed-article:11311349 | pubmed:meshHeading | pubmed-meshheading:11311349... | lld:pubmed |
| pubmed-article:11311349 | pubmed:meshHeading | pubmed-meshheading:11311349... | lld:pubmed |
| pubmed-article:11311349 | pubmed:meshHeading | pubmed-meshheading:11311349... | lld:pubmed |
| pubmed-article:11311349 | pubmed:meshHeading | pubmed-meshheading:11311349... | lld:pubmed |
| pubmed-article:11311349 | pubmed:meshHeading | pubmed-meshheading:11311349... | lld:pubmed |
| pubmed-article:11311349 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11311349 | pubmed:articleTitle | A rapid real-time quantitative polymerase chain reaction for hepatitis B virus. | lld:pubmed |
| pubmed-article:11311349 | pubmed:affiliation | Molecular Biology Unit, Sexually Transmitted and Blood Borne Virus Laboratory, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK. | lld:pubmed |
| pubmed-article:11311349 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11311349 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11311349 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11311349 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11311349 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11311349 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11311349 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11311349 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11311349 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11311349 | lld:pubmed |
