Linked Life Data

11294569

Source:http://linkedlifedata.com/resource/pubmed/id/11294569

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pubmed-article:11294569pubmed:abstractTextDuring the assembly of major histocompatibility complex (MHC) class I molecules transient associations are formed with the endoplasmic reticulum resident chaperones calnexin and calreticulin, ERp57 oxidoreductase, and also with tapasin, the latter mediating binding of the class I molecules to the transporter associated with antigen processing (TAP). We report here the isolation of a cDNA encoding rat tapasin from a DA (RT1av1) library. The cDNA encodes a proline-rich (11.3%) polypeptide of 464 residues with a potential ER-retention KK motif at its COOH-terminus, and a predicted molecular mass of 48 kDa. Matrix-assisted laser-desorption ionisation (MALDI) mass spectrometry of peptides derived from in-gel tryptic digestion of a TAP-associated protein match regions of the predicted translation product. A species of the correct molecular mass and predicted pl was also identified in association with radiolabelled immunoprecipitates of the rat TAP complex analysed by two-dimensional gel electrophoresis. This confirms rat tapasin as a component of the rat MHC class I assembly complex.lld:pubmed
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pubmed-article:11294569pubmed:authorpubmed-author:PowisS JSJlld:pubmed
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pubmed-article:11294569pubmed:authorpubmed-author:ButcherG WGWlld:pubmed
pubmed-article:11294569pubmed:authorpubmed-author:DeversonE VEVlld:pubmed
pubmed-article:11294569pubmed:authorpubmed-author:MorriceN ANAlld:pubmed
pubmed-article:11294569pubmed:authorpubmed-author:HerbertJ RJRlld:pubmed
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pubmed-article:11294569pubmed:pagination48-51lld:pubmed
pubmed-article:11294569pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:11294569pubmed:year2001lld:pubmed
pubmed-article:11294569pubmed:articleTitleRat tapasin: cDNA cloning and identification as a component of the class I MHC assembly complex.lld:pubmed
pubmed-article:11294569pubmed:affiliationMolecular Immunology Programme, The Babraham Institute, Cambridge, UK.lld:pubmed
pubmed-article:11294569pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:11294569pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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