| pubmed-article:11287091 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11287091 | lifeskim:mentions | umls-concept:C0008109 | lld:lifeskim |
| pubmed-article:11287091 | lifeskim:mentions | umls-concept:C0030298 | lld:lifeskim |
| pubmed-article:11287091 | lifeskim:mentions | umls-concept:C0023175 | lld:lifeskim |
| pubmed-article:11287091 | lifeskim:mentions | umls-concept:C0027893 | lld:lifeskim |
| pubmed-article:11287091 | lifeskim:mentions | umls-concept:C1621296 | lld:lifeskim |
| pubmed-article:11287091 | lifeskim:mentions | umls-concept:C1510827 | lld:lifeskim |
| pubmed-article:11287091 | lifeskim:mentions | umls-concept:C1709059 | lld:lifeskim |
| pubmed-article:11287091 | lifeskim:mentions | umls-concept:C0439284 | lld:lifeskim |
| pubmed-article:11287091 | lifeskim:mentions | umls-concept:C1707689 | lld:lifeskim |
| pubmed-article:11287091 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:11287091 | pubmed:dateCreated | 2001-4-5 | lld:pubmed |
| pubmed-article:11287091 | pubmed:abstractText | Neuropeptide Y (NPY) and pancreatic polypeptide (PP) bind to the Y-receptors with very different affinities: NPY has high affinity for the receptors Y(1), Y(2) and Y(5), while PP binds only to Y(4)-receptor with picomolar affinity. By exchanging of specific amino acid positions between the two peptides, we developed 38 full-length PP/NPY chimeras with binding properties that are completely different from those of the two native ligands. Pig NPY (pNPY) analogs containing the segment 19-23 from human PP (hPP) bound to the Y-receptors with much lower affinity than NPY itself. The affinity of the hPP analog containing the pNPY segments 1-7 and 19-23 was comparable to that of pNPY at the Y(1)- and Y(5)-receptor subtypes, and to that of hPP at the Y(4)-receptor. Furthermore, the presence of the segments 1-7 from chicken PP (cPP) and 19-23 from pNPY within the hPP sequence led to a ligand with IC(50) of 40 pM at the Y(5)-receptor. This is the most potent Y(5)-receptor ligand known so far, with 15-fold higher affinity than NPY. | lld:pubmed |
| pubmed-article:11287091 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11287091 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11287091 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11287091 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11287091 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11287091 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11287091 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11287091 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11287091 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:11287091 | pubmed:issn | 0196-9781 | lld:pubmed |
| pubmed-article:11287091 | pubmed:author | pubmed-author:LangerMM | lld:pubmed |
| pubmed-article:11287091 | pubmed:author | pubmed-author:Beck-Sickinge... | lld:pubmed |
| pubmed-article:11287091 | pubmed:author | pubmed-author:WielandH AHA | lld:pubmed |
| pubmed-article:11287091 | pubmed:author | pubmed-author:StidsenC ECE | lld:pubmed |
| pubmed-article:11287091 | pubmed:author | pubmed-author:CabreleCC | lld:pubmed |
| pubmed-article:11287091 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11287091 | pubmed:volume | 22 | lld:pubmed |
| pubmed-article:11287091 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11287091 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11287091 | pubmed:pagination | 365-78 | lld:pubmed |
| pubmed-article:11287091 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:11287091 | pubmed:meshHeading | pubmed-meshheading:11287091... | lld:pubmed |
| pubmed-article:11287091 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11287091 | pubmed:articleTitle | Y-receptor affinity modulation by the design of pancreatic polypeptide/neuropeptide Y chimera led to Y(5)-receptor ligands with picomolar affinity. | lld:pubmed |
| pubmed-article:11287091 | pubmed:affiliation | Department of Applied Bioscience, ETH Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland. | lld:pubmed |
| pubmed-article:11287091 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11287091 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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