| pubmed-article:11277690 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11277690 | lifeskim:mentions | umls-concept:C0206419 | lld:lifeskim |
| pubmed-article:11277690 | lifeskim:mentions | umls-concept:C0596901 | lld:lifeskim |
| pubmed-article:11277690 | lifeskim:mentions | umls-concept:C0002812 | lld:lifeskim |
| pubmed-article:11277690 | lifeskim:mentions | umls-concept:C0599561 | lld:lifeskim |
| pubmed-article:11277690 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:11277690 | pubmed:dateCreated | 2001-3-30 | lld:pubmed |
| pubmed-article:11277690 | pubmed:abstractText | Coronavirus small envelope protein E has two known biological functions: it plays a pivotal role in virus envelope formation, and the murine coronavirus E protein induces apoptosis in E protein-expressing cultured cells. The E protein is an integral membrane protein. Its C-terminal region extends cytoplasmically in the infected cell and in the virion toward the interior. The N-terminal two-thirds of the E protein is hydrophobic and lies buried within the membrane, but its orientation in the lipid membrane is not known. Immunofluorescent analyses of cells expressing biologically active murine coronavirus E protein with a hydrophilic short epitope tag at the N-terminus showed that the epitope tag was exposed cytoplasmically. Immunoprecipitation analyses of the purified microsomal membrane vesicles that contain the same tagged E protein revealed the N-terminal epitope tag outside the microsomal membrane vesicles. These analyses demonstrated that the epitope tag at the N-terminus of the E protein was exposed cytoplasmically. Our data were consistent with an E protein topology model, in which the N-terminal two-thirds of the transmembrane domain spans the lipid bilayer twice, exposing the C-terminal region to the cytoplasm or virion interior. | lld:pubmed |
| pubmed-article:11277690 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11277690 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11277690 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11277690 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11277690 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11277690 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11277690 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:11277690 | pubmed:issn | 0042-6822 | lld:pubmed |
| pubmed-article:11277690 | pubmed:author | pubmed-author:MaedaAA | lld:pubmed |
| pubmed-article:11277690 | pubmed:author | pubmed-author:MakinoSS | lld:pubmed |
| pubmed-article:11277690 | pubmed:author | pubmed-author:MaedaJJ | lld:pubmed |
| pubmed-article:11277690 | pubmed:author | pubmed-author:RepassJ FJF | lld:pubmed |
| pubmed-article:11277690 | pubmed:copyrightInfo | Copyright 2001 Academic Press. | lld:pubmed |
| pubmed-article:11277690 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11277690 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:11277690 | pubmed:volume | 281 | lld:pubmed |
| pubmed-article:11277690 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11277690 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11277690 | pubmed:pagination | 163-9 | lld:pubmed |
| pubmed-article:11277690 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:11277690 | pubmed:meshHeading | pubmed-meshheading:11277690... | lld:pubmed |
| pubmed-article:11277690 | pubmed:meshHeading | pubmed-meshheading:11277690... | lld:pubmed |
| pubmed-article:11277690 | pubmed:meshHeading | pubmed-meshheading:11277690... | lld:pubmed |
| pubmed-article:11277690 | pubmed:meshHeading | pubmed-meshheading:11277690... | lld:pubmed |
| pubmed-article:11277690 | pubmed:meshHeading | pubmed-meshheading:11277690... | lld:pubmed |
| pubmed-article:11277690 | pubmed:meshHeading | pubmed-meshheading:11277690... | lld:pubmed |
| pubmed-article:11277690 | pubmed:meshHeading | pubmed-meshheading:11277690... | lld:pubmed |
| pubmed-article:11277690 | pubmed:meshHeading | pubmed-meshheading:11277690... | lld:pubmed |
| pubmed-article:11277690 | pubmed:meshHeading | pubmed-meshheading:11277690... | lld:pubmed |
| pubmed-article:11277690 | pubmed:meshHeading | pubmed-meshheading:11277690... | lld:pubmed |
| pubmed-article:11277690 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11277690 | pubmed:articleTitle | Membrane topology of coronavirus E protein. | lld:pubmed |
| pubmed-article:11277690 | pubmed:affiliation | Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, Texas 77555, USA. | lld:pubmed |
| pubmed-article:11277690 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11277690 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:11277690 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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